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Anti-Mouse TGFBI/BIGH3 Rabbit Recombinant Antibody, PBS Only (98667-2-PBS)

Anti-Mouse TGFBI/BIGH3 Rabbit Recombinant Antibody, PBS Only

Name: Anti-Mouse TGFBI/BIGH3 Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98667-2-PBS-1MG

TGFBI/BIGH3 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98667-2-PBS

Clone No.251191C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Beta ig-h3, BIGH3, Tgfbi, Transforming growth factor-beta-induced protein ig-h3, βIG-H3

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllFC (Intra)Affinity

FC experiment of Transfected HEK-293T using 98667-2-RR (same clone as 98667-2-PBS)
1x10^6 HEK-293T cells (left) or Tgfbi-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse TGFBI/BIGH3 Rabbit RecAb (98667-2-RR, Clone:251191C5), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98667-2-PBS in a different storage buffer formulation.
×
1x10^6 HEK-293T cells (left) or Tgfbi-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse TGFBI/BIGH3 Rabbit RecAb (98667-2-RR, Clone:251191C5), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98667-2-PBS in a different storage buffer formulation.
FC experiment of Transfected HEK-293T using 98667-2-RR (same clone as 98667-2-PBS)
1x10^6 HEK-293T cells(left) or Tgfbi-transfected HEK-293T cells(right) were intracellularly stained with 0.25 ug Anti-Mouse Tgfbi Rabbit RecAb (98667-2-RR, Clone:251191C5) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98667-2-PBS in a different storage buffer formulation.
×
1x10^6 HEK-293T cells(left) or Tgfbi-transfected HEK-293T cells(right) were intracellularly stained with 0.25 ug Anti-Mouse Tgfbi Rabbit RecAb (98667-2-RR, Clone:251191C5) (red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 98667-2-PBS in a different storage buffer formulation.
Affinity of 98667-2-RR
Biolayer interferometry (BLl) kinetic assays of 98667-2-RR against Mouse TGFBI/BIGH3 were performed. The affinity constant is 1.52 nM.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98667-2-PBS targets TGFBI/BIGH3 in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3160 Product name: Recombinant Mouse TGFBI/BIGH3 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-683 aa of NM_009369.4 Sequence: GPAKSPYQLVLQHSRLRGRQHGPNVCAVQKVIGTNKKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETMGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWSSLPAEVLDSLVSNVNIELLNALRYHMVDRRVLTDELKHGMTLTSMYQNSNIQIHHYPNGIVTVNCARLLKADHHATNGVVHLIDKVISTITNNIQQIIEIEDTFETLRAAVAASGLNTVLEGDGQFTLLAPTNEAFEKIPAETLNRILGDPEALRDLLNNHILKSAMCAEAIVAGMSMETLGGTTLEVGCSGDKLTINGKAVISNKDILATNGVIHFIDELLIPDSAKTLLELAGESDVSTAIDILKQAGLDTHLSGKEQLTFLAPLNSVFKDGVPRIDAQMKTLLLNHMVKEQLASKYLYSGQTLDTLGGKKLRVFVYRNSLCIENSCIAAHDKRGRFGTLFTMDRMLTPPMGTVMDVLKGDNRFSMLVAAIQSAGLMEILNREGVYTVFAPTNEAFQAMPPEELNKLLANAKELTNILKYHIGDEILVSGGIGALVRLKSLQGDKLEVSSKNNVVSVNKEPVAETDIMATNGVVYAINTVLQPPANRPQERGDELADSALEIFKQASAYSRAAQRSVRLAPVYQRLLERMKH Predict reactive species
Full Name	transforming growth factor, beta induced
Calculated Molecular Weight	75 kDa
GenBank Accession Number	NM_009369.4
Gene Symbol	Tgfbi
Gene ID (NCBI)	21810
RRID	AB_3746258
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P82198
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

TGFBI, also named as BIGH3, Kerato-epithelin and RGD-CAP, binds to type I, II, and IV collagens. TGFBI is an adhesion protein which may play an important role in cell-collagen interactions. In cartilage, it may be involved in endochondral bone formation. TGFBI is an extracellular matrix adaptor protein, it has been reported to be differentially expressed in transformed tissues. TGFBI is a predictive factor of the response to chemotherapy, and suggest the use of TGFBI-derived peptides as possible therapeutic adjuvants for the enhancement of responses to chemotherapy.(PMID:20509890) Defects in TGFBI are the cause of epithelial basement membrane corneal dystrophy (EBMD). Defects in TGFBI are the cause of corneal dystrophy Groenouw type 1 (CDGG1). Defects in TGFBI are the cause of corneal dystrophy lattice type 1 (CDL1). Defects in TGFBI are a cause of corneal dystrophy Thiel-Behnke type (CDTB). Defects in TGFBI are the cause of Reis-Buecklers corneal dystrophy (CDRB). Defects in TGFBI are the cause of lattice corneal dystrophy type 3A (CDL3A). Defects in TGFBI are the cause of Avellino corneal dystrophy (ACD).