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CoraLite® Plus 488-conjugated TGM2 Monoclonal antibody

TGM2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2D4C11

Cat no : CL488-68006

Synonyms

G ALPHA h, GNAH, TG(C), TG2, TGase 2, TGase C, TGase H, TGC, TGM2, Tissue transglutaminase, Transglutaminase 2, Transglutaminase C, Transglutaminase H

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated TGM2 Monoclonal anti (CL488-68006)

    FC experiment of HeLa using CL488-68006

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TGM2 (CL488-68006, Clone:2D4C11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68006 targets TGM2 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TGM2 fusion protein Ag7462
Full Name transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
Calculated Molecular Weight 77 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC003551
Gene Symbol TGM2
Gene ID (NCBI) 7052
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TGM2 antibody CL488-68006Download protocol
Standard Protocols
Click here to view our Standard Protocols