TINAGL1 antibody (12077-1-PBS)

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis using 12077-1-AP (same clone as 12077-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 12077-1-AP (same clone as 12077-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
WB analysis of human adrenal gland using 12077-1-AP (same clone as 12077-1-PBS)
human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
IP experiment of mouse heart using 12077-1-AP (same clone as 12077-1-PBS)
IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 12077-1-AP (same clone as 12077-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12077-1-AP (same clone as 12077-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TINAGL1 antibody (12077-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 12077-1-PBS in a different storage buffer formulation.

This product is currently not available for sale.

Tested Applications
Product Information

Product Information

12077-1-PBS targets TINAGL1 in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TINAGL1 fusion protein Ag2721 Predict reactive species
Full Name	tubulointerstitial nephritis antigen-like 1
Calculated Molecular Weight	467 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC009048
Gene Symbol	TINAGL1
Gene ID (NCBI)	64129
RRID	AB_2058942
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9GZM7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.