TINAGL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TINAGL1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 12077-1-AP

Synonyms

ARG1, AZ1, GIS5, LCN7, LIECG3, OLRG 2, OLRG2, PSEC0088, TIN Ag related protein, TIN Ag RP, TINAGL, TINAGL1, TINAGRP, UNQ204/PRO230

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Validation Data Gallery

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  WB analysis using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12077-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 12077-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 12077-1-AP (TINAGL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 12077-1-AP

  IP result of anti-TINAGL1 (IP:12077-1-AP, 4ug; Detection:12077-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12077-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12077-1-AP (TINAGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 12077-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TINAGL1 antibody (12077-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human placenta tissue, human adrenal gland tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human liver cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1500
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

12077-1-AP targets TINAGL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TINAGL1 fusion protein Ag2721
• Full Name: tubulointerstitial nephritis antigen-like 1
• Calculated Molecular Weight: 467 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC009048
• Gene Symbol: TINAGL1
• Gene ID (NCBI): 64129
• RRID: AB_2058942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulointerstitial nephritis antigen-like 1 (Tinagl1), a secreted extracellular protein, was initially identified as a putative component of the ECM. Tinagl1 is most commonly associated with embryonic development, including the heart and adrenal glands. Low levels of Tinagl1 expression can be dire for craniofacial development and for female fertility.

Publications

Species	Application	Title
human,mouse	WB,IHC	FASEB J; Down-regulated TINAGL1 in fibroblasts impairs wound healing in diabetes.; Authors - Wen-Qing Tian
human,mouse	WB	Biochim Biophys Acta Mol Cell Res; Functional analysis reveals that Tinagl1 is required for normal muscle development in mice through the activation of ERK signaling.; Authors - Yoriko Sato ; KD Validated
human	WB,IP	J Transl Med; Tubulointerstitial nephritis antigen-like 1 from cancer-associated fibroblasts contribute to the progression of diffuse-type gastric cancers through the interaction with integrin β1; Authors - Dagyeong Lee
human	WB	Cancer Cell; Tinagl1 Suppresses Triple-Negative Breast Cancer Progression and Metastasis by Simultaneously Inhibiting Integrin/FAK and EGFR Signaling.; Authors - Minhong Shen
human	WB	Cancer Manag Res; TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression.; Authors - Lu Sun ; KD Validated