Product Information
24839-1-PBS targets TNN in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18127 Product name: Recombinant human TNN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 713-1062 aa of BC136619 Sequence: NLVTDRVTENMATVSWDPVRATIDRYVVRYTSAKDGETREVPVGKEQSSTVLTGLRPGVEYTVHVWAQKGAQESKKADTKAQTDIDSPQNLVTDWVTENTATVSWDPVQATIDRYVVHYTSANGETREVPVGKEQSSTVLTGLRPGMEYTVHVWAQKGNQESKKADTKAQTEIDGPKNLVTDWVTENMATVSWDPVQATIDKYMVRYTSADGETREVPVGKEHSSTVLTGLRPGMEYMVHVWAQKGAQESKKADTKAQTELDPPRNLRPSAVTQSGGILTWTPPSAQIHGYILTYQFPDGTVKEMQLGREDQRFALQGLEQGATYPVSLVAFKGGRRSRNVSTTLSTVGA Predict reactive species |
| Full Name | tenascin N |
| Calculated Molecular Weight | 1299 aa, 144 kDa |
| Observed Molecular Weight | 144 kDa, 173 kDa |
| GenBank Accession Number | BC136619 |
| Gene Symbol | TNN |
| Gene ID (NCBI) | 63923 |
| RRID | AB_3669454 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQP3 |
| Storage Buffer | PBS only, pH 7.3. |
| Storage Conditions | Store at -80°C. |
Background Information
TNN (Tenascin-W), also known as TNW. It is expected to be located in the extracellular space. The protein is not detected in normal adult mammary tissues or brain but expressed in most breast tumors and brain tumors, such as glioblastomas, astrocytomas and oligodendrogliomas, tested (PMID:17909022). And in brain tumors, detected around the endothelial cell layer of the clood vessels (PMID: 19884327). TNN is the fourth and final member of the extracellular matrix protein family of tenascins. In vitro, the presence of tenascin-W increased the proportion of elongated human umbilical vein endothelial cells (HUVECs) and augmented the mean speed of cell migration. Furthermore, tenascin-W triggered sprouting of HUVEC spheroids to a similar extent as the proangiogenic factor tenascin-C. Tenascin-W is a specific marker of glioma-associated blood vessels and stimulates angiogenesis in vitro(PMID: 19884327). The predicted molecular weight of the protein is 144 kDa in human and 173 kDa in mouse.
