Tested Applications
| Positive WB detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31990-1-AP targets TRIM65 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34639 Product name: Recombinant human TRIM65 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 320-517 aa of NM_173547 Sequence: CPLRRKLWQNYRNLTFDPVSANRHFYLSRQDQQVKHCRQSRGPGGPGSFELWQVQCAQSFQAGHHYWEVRASDHSVTLGVSYPQLPRCRLGPHTDNIGRGPCSWGLCVQEDSLQAWHNGEAQRLPGVSGRLLGMDLDLASGCLTFYSLEPQTQPLYTFHALFNQPLTPVFWLLEGRTLTLCHQPGAVFPLGPQEEVLS Predict reactive species |
| Full Name | tripartite motif-containing 65 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 57 kDa, 70 kDa |
| GenBank Accession Number | NM_173547 |
| Gene Symbol | TRIM65 |
| Gene ID (NCBI) | 201292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q6PJ69 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tripartite motif-containing protein 65 (TRIM65) is a member of the TRIM family of RING-type E3 ubiquitin ligases. It contains a RING-finger domain, a B-box domain, a coiled-coil domain, and a SPRY domain (PMID: 40473847). As an E3 ubiquitin ligase, TRIM65 is involved in the ubiquitination of substrate proteins, playing a role in various intracellular physiological processes, including intracellular signaling, protein degradation, cell proliferation, apoptosis, carcinogenesis, autophagy, and phenotypic transformation (PMID: 38939997). TRIM65 has been shown to promote ubiquitination and degradation of several proteins, such as TOX4, which is involved in apoptosis, and ARHGAP35, which is involved in cell migration (PMID: 38212319; 31332286). The 70 kDa band might be the result of SUMO modification.
