TRUB1 Recombinant antibody

TRUB1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85746-1-RR
Clone No.243129F4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

EC:5.4.99.-, EC:5.4.99.25, Pseudouridylate synthase TRUB1, Psi55 synthase TRUB1, PUS4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HEK-293T cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85746-1-RR targets TRUB1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TRUB1 fusion protein Ag3213 Predict reactive species
Full Name TruB pseudouridine (psi) synthase homolog 1 (E. coli)
Calculated Molecular Weight 349 aa, 37 kDa
Observed Molecular Weight 36 kDa, 39 kDa
GenBank Accession NumberBC030601
Gene Symbol TRUB1
Gene ID (NCBI) 142940
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WWH5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRUB1 gene encodes a 349-amino acid product, with a VFAVHKPKGPTSA box in positions 71-83 corresponding to motif I of the TruB family (probably involved in conserving protein structure). This protein may be responsible for synthesis of pseudouridine from uracil in transfer RNAs.

Protocols

Product Specific Protocols
WB protocol for TRUB1 antibody 85746-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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