Tested Applications
| Positive WB detected in | A549 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15314-1-AP targets TSPAN14 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7551 Product name: Recombinant human TSPAN14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 97-219 aa of BC002920 Sequence: LFQDWVRDRFREFFESNIKSYRDDIDLQNLIDSLQKANQCCGAYGPEDWDLNVYFNCSGASYSREKCGVPFSCCVPDPAQKVVNTQCGYDVRIQLKSKWDESIFTKGCIQALESWLPRNIYIV Predict reactive species |
| Full Name | tetraspanin 14 |
| Calculated Molecular Weight | 31 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC002920 |
| Gene Symbol | TSPAN14 |
| Gene ID (NCBI) | 81619 |
| RRID | AB_2918039 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NG11 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TSPAN14 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN14 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPAN14 antibody 15314-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TSPAN14 Polyclonal antibody (catalog number 15314-1-AP) has 1 total citation. It appears in the journal Journal of Extracellular Vesicles (2024, impact factor 21.7). The associated research field is extracellular vesicle biology, specifically profiling high-purity human blood extracellular vesicles using AF4 combined with density cushion ultracentrifugation, with the antibody applied in Western blot (WB) for human samples.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Optimized AF4 combined with density cushion ultracentrifugation enables profiling of high-purity human blood extracellular vesicles |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot on Jurkat cells and reported that the antibody performs well at the dilution recommended by the supplier. Overall, the feedback is positive, confirming reliable performance under the manufacturer's suggested conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jean-Baptiste (Verified Customer) (09-13-2026) | The antibody works at the dilution specified by the supplier in a Western blot
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