Filter: AllWBIF/ICCAffinity

WB analysis using 87282-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 87282-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 87282-1-RR (UBE2O antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IF Staining of A431 using 87282-1-RR
Immunofluorescent analysis of (4% PFA) fixed A431 cells using UBE2O antibody (87282-1-RR, Clone: 252545E2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Affinity of 87282-1-RR
Biolayer interferometry (BLl) kinetic assays of 87282-1-RR against Human UBE2O were performed. The affinity constant is 16.1 nM.

UBE2O Recombinant monoclonal antibody

Name: UBE2O Recombinant monoclonal antibody
Brand: Proteintech
SKU: 87282-1-RR

UBE2O Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 87282-1-RR

Clone No.252545E2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

(E3-independent) E2 ubiquitin-conjugating enzyme, E2/E3 hybrid ubiquitin-protein ligase UBE2O, E2-230K, EC:2.3.2.24, KIAA1734

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, rat brain tissue, Jurkat cells, HCT 116 cells, mouse brain tissue
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

87282-1-RR targets UBE2O in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag8580 Product name: Recombinant human UBE2O protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-343 aa of BC007822 Sequence: SSDSGPEAGSQRLLFSHDLVSGRYRGSVHFGLVRLIHGEDSDSEGEEEGRGSSGCSEAGGAGHEEGRASPLRRGYVRVQWYPEGVKQHVKETKLKLEDRSVVPRDVVRHMRSTDSQCGTVIDVNIDCAVKLIGTNCIIYPVNSKDLQHIWPFMYGDYIAYDCWLGKVYDLKNQIILKLSNGARCSMNTEDGAKLYDVCPHVSDSGLFFDDSYGFYPGQVLIGPAKIFSSVQWLSGVKPVLSTKSKFRVVVEEVQVVELKVTWITKSFCPGGTDSVSPPPSVITQENLGRVKRLGCFDHAQRQLGERCLYVFPAKVEPAKIAWECPEKNCAQGEGSMAKKVKRL Predict reactive species
Full Name	ubiquitin-conjugating enzyme E2O
Calculated Molecular Weight	1292 aa, 141 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC007822
Gene Symbol	UBE2O
Gene ID (NCBI)	63893
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9C0C9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2O, also named as KIAA1734, belongs to the ubiquitin-conjugating enzyme family. It catalyzes the covalent attachment of ubiquitin to other proteins. UBE2O has a calculated molecular mass of 141 kDa, and many bands can be detected as 140-170 kDa and 200-230 kDa (PMID: 28774900, 23455153, 28774922, 11311559).