UGT8 Recombinant antibody

UGT8 Recombinant Antibody for FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC, ELISA

Conjugate

Unconjugated

CloneNo.

230107A6

Cat no : 82874-1-RR

Synonyms

Cerebroside synthase, CGT, UDP glycosyltransferase 8, UGT4, UGT8

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Validation Data Gallery

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  FC experiment of HeLa using 82874-1-RR

  1X10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human UGT8 (82874-1-RR, Clone:230107A6) and Alexa Fluor™ Plus 647-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82874-1-RR targets UGT8 in FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UGT8 fusion protein Ag13137
• Full Name: UDP glycosyltransferase 8
• Calculated Molecular Weight: 541 aa, 61 kDa
• GenBank Accession Number: BC075069
• Gene Symbol: UGT8
• Gene ID (NCBI): 7368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UGT8, also known as galacotosylceramide synthase or CGT (ceramide galactosyltransferase), is a key enzyme for galacotosylceramide (GalCer) biosynthesis. It is an ER transmembrane protein and has limited tissue distribution, with predominance in Schwann cells, oligodendrocytes, kidneys, testes, and intestine. UGT8 is highly enriched in myelin in the central nervous system. Its expression levels are strongly associated with histological typing in human oligodendrogliomas and astrocytomas; can be used as molecular marker to distinguish these tumors. Higher UGT8 levels had also been linked to metastatic properties of cancer cells.