USP7 Monoclonal antibody

• Featured Product
• KD/KO Validated

USP7 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

CloneNo.

3D7D11

Cat no : 66514-1-Ig

Synonyms

Deubiquitinating enzyme 7, HAUSP, TEF1, Ubiquitin thioesterase 7, USP7

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Validation Data Gallery

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  WB analysis using 66514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66514-1-Ig

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected HeLa cells.

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  WB analysis of A549 using 66514-1-Ig

  WB result of USP7 antibody (66514-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP7 transfected A549 cells.

• 

  WB analysis using 66514-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66514-1-Ig (USP7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66514-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66514-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66514-1-Ig (USP7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 66514-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66514-1-Ig (USP7 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, LNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, ROS1728 cells, NIH/3T3 cells, HepG2 cells, K-562 cells, 4T1 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:20000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 4 publications below

Product Information

66514-1-Ig targets USP7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: USP7 fusion protein Ag25652
• Full Name: ubiquitin specific peptidase 7 (herpes virus-associated)
• Observed molecular weight: 128 kDa
• Gene symbol: USP7
• Gene ID (NCBI): 7874
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IP	Oncogene; The deubiquitinase USP7 regulates oxidative stress through stabilization of HO-1.; Authors - Ming Gao ; KD Validated
human	WB	EBioMedicine; Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.; Authors - Zhen Yu
human	WB	J Biomed Sci; TRIM21-SERPINB5 aids GMPS repression to protect nasopharyngeal carcinoma cells from radiation-induced apoptosis.; Authors - Panpan Zhang
human	WB,IP	J Cell Sci; The role of USP7 in the Shoc2-ERK1/2 signaling axis and Noonan-like syndrome with loose anagen hair.; Authors - Patricia Wilson ; KD Validated
mouse	WB	Mol Cells; BK Channel Deficiency in Osteoblasts Reduces Bone Formation via the Wnt/β-Catenin Pathway.; Authors - Lan Jiang
human	WB,IP	Mol Oncol; Deubiquitination of FBP1 by USP7 blocks FBP1-DNMT1 interaction and decreases the sensitivity of pancreatic cancer cells to PARP inhibitors; Authors - Xiang Cheng ; KD Validated