Rat VCAM-1/CD106 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86797-1-RR (same clone as 86797-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86797-1-RR (Vcam1/CD106 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86797-1-PBS in a different storage buffer formulation.

VCAM-1/CD106 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86797-1-PBS

Clone No.251266C11

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, Indirect ELISA

Synonyms

CD106, Soluble Vascular Cell Adhesion Molecule-1, Vascular cell adhesion protein 1, V-CAM 1, Vcam1

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Product Information

86797-1-PBS targets VCAM-1/CD106 in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3821

  Product name: Recombinant Rat VCAM-1/CD106 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 25-698 aa of NM_012889.1

  Sequence: FKIEISPEYKTLAQIGDSMLLTCSTTGCESPSFSWRTQIDSPLNGKVKTEGAKSVLTMDPVSFENEHSYLCTATCNSGKLERGIQVDIYSFPKDPEIQFSGPLEVGKPVMVKCLAPDVYPIDRLEIELFKGDRLMKKQDFVDEMAKKSLETKSLEVIFTPVIEDIEKALVCRAKLYIDQTDSIPKERETVRELQVYTSPKNTEISVHPSTRLHEGAAVTMTCASEGLPAPEIFWSKKLDNGVLQLLSGNATLTLIAMRMEDSGIYVCEGVNLVGRDKTEVELIVQEKPFTVDISPGSQVAAQVGDSVVLTCAAVGCDSPSFSWRTQTDSPLNGEVRDEGATSTLTLSPVGVEDEHSYLCTVTCQRRKLEKTIQVEVYSFPEDPEIEISGPLVHGRPVTVNCTVPNVYPFDHLEIELLKGETTLLNKFLREEIGTKSLETKSLEMTFIPTAEDTGKALVCLAKLHSSQMESEPKQRQSTQTLYVNVAPKEPTIWVSPSPVPEEGSPVNLTCSSDGFPTPKILWSRQLKNGELQPLSQNTTLSFMATKMEDSGIYVCEGINEAGISKKSVELIIQGSSKDIQLTVFPSKSVKEGDTVIISCTCGSVPEIWIILKKKAKTGDMVLKSVNGSYTIRKAQLQDAGVYECESKTEVGSQLRSLTLDVKGKENNKDYFSPE

• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: NM_012889.1
• Gene Symbol: Vcam1
• Gene ID (NCBI): 25361
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29534
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a 110-kDa transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).