CoraLite® Plus 488-conjugated VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for IF/ICC
Cat No. CL488-67590
Clone No.2E10G11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC

hVPS18, KIAA1475, PEP3, VPS18

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67590 targets VPS18 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29954

Product name: Recombinant human VPS18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 226-355 aa of BC001513

Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVL

Predict reactive species
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated Molecular Weight 110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2919503
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VPS18 antibody CL488-67590Download protocol
Standard Protocols
Click here to view our Standard Protocols
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