Tested Applications
| Positive IF/ICC detected in | A549 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-67590 targets VPS18 in IF/ICC applications and shows reactivity with Human samples.
| Tested Reactivity | Human | 
| Host / Isotype | Mouse / IgG2a | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag29954 Product name: Recombinant human VPS18 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 226-355 aa of BC001513 Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLPredict reactive species | 
| Full Name | vacuolar protein sorting 18 homolog (S. cerevisiae) | 
| Calculated Molecular Weight | 110 kDa | 
| GenBank Accession Number | BC001513 | 
| Gene Symbol | VPS18 | 
| Gene ID (NCBI) | 57617 | 
| RRID | AB_2919503 | 
| Conjugate | CoraLite® Plus 488 Fluorescent Dye | 
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm | 
| Form | Liquid | 
| Purification Method | Protein A purification | 
| UNIPROT ID | Q9P253 | 
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. | 
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Background Information
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VPS18 antibody CL488-67590 | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 


