VPS18 Monoclonal antibody

VPS18 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67590-1-Ig
Clone No.2E10G11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat

Applications

WB, IHC, IF/ICC, ELISA

hVPS18, KIAA1475, PEP3, VPS18

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67590-1-Ig targets VPS18 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Reactivity Human, Rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag29954

Product name: Recombinant human VPS18 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 226-355 aa of BC001513

Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVL

Predict reactive species
Full Name vacuolar protein sorting 18 homolog (S. cerevisiae)
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC001513
Gene Symbol VPS18
Gene ID (NCBI) 57617
RRIDAB_2882798
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9P253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

Protocols

Product Specific Protocols
WB protocol for VPS18 antibody 67590-1-IgDownload protocol
IHC protocol for VPS18 antibody 67590-1-IgDownload protocol
IF protocol for VPS18 antibody 67590-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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