Vimentin Monoclonal antibody, PBS Only (Detector)

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  WB analysis of Jurkat cells using 60330-1-Ig (same clone as 60330-1-PBS)

  WB result of Vimentin antibody (60330-1-Ig, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  WB analysis using 60330-1-Ig (same clone as 60330-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 60330-1-Ig (same clone as 60330-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 60330-1-Ig (same clone as 60330-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 60330-1-Ig (Vimentin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP50158-1

  The mean Vimentin concentration was determined to be 3,886.65 ng/mL in A549 cell extract based on a 2.6 mg/mL extract load.

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  Sandwich ELISA standard curve of MP50158-1

  Sandwich ELISA standard curve of MP50158-1, Human Vimentin Monoclonal Matched Antibody Pair - PBS only. 60330-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0489. 60330-1-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

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  IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:102400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human renal cell carcinoma using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 60330-1-Ig (Vimentin Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60330-1-Ig (Vimentin antibody) at dilution of 1:6400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 60330-1-Ig (Vimentin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 60330-1-Ig (same clone as 60330-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using Vimentin antibody (60330-1-Ig, Clone: 3H9D1 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  IF Staining of Retinal organoids using 60330-1-Ig (same clone as 60330-1-PBS)

  Retinal organoids (day 250) generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for Vimentin with 60330-1-Ig at 1:500 dilution (green) and Caspase 3 using 19677-1-AP at 1:400 (red). Nuclear stain DAPI (blue). Scale bar = 20 µm. Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria. This data was developed using the same antibody clone with 60330-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50158-1

  Cytometric bead array standard curve of MP50158-1, Vimentin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60330-2-PBS. Detection antibody: 60330-1-PBS. Standard:Ag0489. Range: 3.125-100 ng/mL.

• Featured Product
• KD/KO Validated

Vimentin Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 60330-1-PBS

Clone No.3H9D1

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

VIM, 3H9D1

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Published Applications

• IF: See 1 publications below

Product Information

60330-1-PBS targets Vimentin as part of a matched antibody pair:

MP50158-1: 60330-2-PBS capture and 60330-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: goat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0489

  Product name: Recombinant human Vimentin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-466 aa of BC000163

  Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• ENSEMBL Gene ID: ENSG00000026025
• RRID: AB_2881439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P08670
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
goat	IF	Int J Nanomedicine; Graphene Oxide Potentiates Induction Efficiency and Functional Maturation of Induced Mammary Epithelial Cells.; Authors - Zhigang Lei