"YY1 Antibodies" Comparison
View side-by-side comparison of YY1 antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | C6 cells, A431 cells, COS-7 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66281-1-Ig targets YY1 in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
| Tested Reactivity | human, mouse, rat, monkey |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17732 Product name: Recombinant human YY1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-414 aa of BC037308 Sequence: MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGGSSSSGGGRVKKGGGKKSGKKSYLSGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ Predict reactive species |
| Full Name | YY1 transcription factor |
| Calculated Molecular Weight | 414 aa, 45 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | BC037308 |
| Gene Symbol | YY1 |
| Gene ID (NCBI) | 7528 |
| RRID | AB_2881664 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P25490 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-70 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for YY1 antibody 66281-1-Ig | Download protocol |
| IHC protocol for YY1 antibody 66281-1-Ig | Download protocol |
| IP protocol for YY1 antibody 66281-1-Ig | Download protocol |
| WB protocol for YY1 antibody 66281-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
YY1 Monoclonal antibody (Cat# 66281-1-Ig) has 73 citations across journals including Cell, Nature Communications, Nature Aging, Cancer Research, Cell Death & Disease, Advanced Science, Oncogene, Genome Biology, PLoS Pathogens, and International Journal of Molecular Sciences. Research fields span oncology (breast, colorectal, lung, pancreatic, prostate, ovarian, melanoma, glioblastoma), cardiovascular disease, epigenetics, immunology, metabolism, neurology, liver fibrosis, infectious disease, and developmental biology.
| Species | Application | Title |
|---|---|---|
Cell Pervasive Chromatin-RNA Binding Protein Interactions Enable RNA-Based Regulation of Transcription. | ||
Mol Cancer CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p. | ||
Nat Aging A Treg-specific long noncoding RNA maintains immune-metabolic homeostasis in aging liver | ||
Cancer Res PRODH2-Mediated Metabolism in the Bone Microenvironment Promotes Breast Cancer Metastasis. | ||
J Extracell Vesicles Salvianolic Acid B Upregulates miR-744-5p Expression in Peripheral Blood Mononuclear Cell-Derived Extracellular Vesicles to Alleviate Uremic Cardiomyopathy. | ||
