ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104]
Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
Cat no : sms1AF488-1
Synonyms
Validation Data Gallery
Product Information
Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
Applications | IF, WB, FC |
Clonality | Biclonal: mixture of 2 monoclonal Nanobodies |
Recommended Dilution | Immunofluorescence 1:500 Super-resolution microscopy 1:500 Western blot 1:1,000 Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000. Note: Image acquisition time may have to optimized |
Host | Alpaca |
Affinity | CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM |
Specificity/Target | Mouse IgG1; this Nanobody mixture recognizes Fc fragments of mouse IgG1 |
Reactivity | Mouse |
GeneID (NCBI) | 0 |
Conjugate | Alexa Fluor® 488 |
Storage Buffer | 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide |
Storage Condition | Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles. |
Documentation
SDS |
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SDS Alpaca anti-mouse IgG1 Alexa Fluor 488 (PDF) |
Protocol |
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Protocol Alpaca anti-mouse IgG1, Fc-specific recombinant VHH, Alexa Fluor 488 (PDF) |
Application note |
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Blocking recommendation for immunostaining with Nano-Secondaries (PDF) |
One-step incubation with Nano-Secondaries (PDF) |
Brochure |
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Chromotek nanobodies brochure (PDF) |