ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104]

Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

Reactivity

Mouse

Applications

IF, WB, FC

Conjugate

Alexa Fluor® 488

Cat no : sms1AF488-1

Synonyms

mouse IgG1; mouse immunoglobulin Gamma 1

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Validation Data Gallery

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  Anti-mouse IgG1 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG1 antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG1 primary antibody.

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  The anti-mouse IgG1 Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG2b and IgG3 subclasses.

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  Vimentin in HeLa cells: The cells were stained with monoclonal anti-Vimentin mouse IgG1 antibody and alpaca anti-mouse IgG1 VHH Alexa Fluor? 488 and recorded with Leica GSDIM system. The image is a courtesy of Dr. Leila Nahidiazar, Dr. Jop Kind, and Prof. Kees Jalink.

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  One-step immunostaining is the simultaneous incubation of mouse IgG1 primary antibody and anti-mouse IgG1 Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

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  Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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Product Information

Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

• Applications: IF, WB, FC
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Recommended Dilution: Immunofluorescence 1:500; Super-resolution microscopy 1:500; Western blot 1:1,000; Optimal working concentration is application-dependent and should be determined by testing a dilution range from 1:250 to 1:2,000.; Note: Image acquisition time may have to optimized
• Host: Alpaca
• Affinity: CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM
• Specificity/Target: Mouse IgG1; this Nanobody mixture recognizes Fc fragments of mouse IgG1
• Reactivity: Mouse
• GeneID (NCBI): 0
• Conjugate: Alexa Fluor® 488
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.