ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488 [CTK0103, CTK0104]

Validation Data Gallery

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  Anti-mouse IgG1 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG1 antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG1 primary antibody.

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  The anti-mouse IgG1 Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG2b and IgG3 subclasses.

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  Vimentin in HeLa cells: The cells were stained with monoclonal anti-Vimentin mouse IgG1 antibody and alpaca anti-mouse IgG1 VHH Alexa Fluor® 488 and recorded with Leica GSDIM system. The image is a courtesy of Dr. Leila Nahidiazar, Dr. Jop Kind, and Prof. Kees Jalink.

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  One-step immunostaining is the simultaneous incubation of mouse IgG1 primary antibody and anti-mouse IgG1 Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

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  Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

Cat No. sms1AF488-1

Publications(8)

Reviews (1)

Applications

IF, WB, FC

Conjugate

Alexa Fluor® 488

Host

Alpaca

Reactivity

Mouse, IgG1

Synonyms

mouse IgG1; mouse immunoglobulin Gamma 1

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Product Information

Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
• Applications: IF, WB, FC
• Host: Alpaca
• Species Reactivity: Mouse IgG1 Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3
• Target/Specificity: This Nanobody mixture recognizes the Fc fragment of mouse IgG1.
• Physical State: Liquid
• Conjugate: Alexa Fluor® 488
• Suggested Dilution: Immunofluorescence 1:500; Super-resolution microscopy 1:500; Western blot 1:1,000
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Mixture of 2 monoclonal Nanobodies; Secondary Nanobody
• Class: Recombinant
• Format: Alpaca single domain antibodies, monovalent
• Cross-reactivity: No cross-reactivity to rabbit, rat, sheep, goat, guinea pig, human, and macaque serum
• Immunogen: Purified mouse IgG1
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Clones: VHH0302, VHH0305
• RRID: AB_2827579
• Affinity (KD): CTK0103: K_D =0.13 nM, CTK0104: K_D = 0.63nM
• Excitation/ Emission: Excitation max: 490 nm, Emission max: 525 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
• Size: 10 µL; 100 µL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Biomater Res; Bioactive self-healing hydrogel based on tannic acid modified gold nano-crosslinker as an injectable brain implant for treating Parkinson's disease; Authors - Junpeng Xu
IF	Int J Biol Macromol; zwitterionic Pluronic analog-coated PLGA nanoparticles for oral insulin delivery; Authors - Kedong Liu
WB	iScience; Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress; Authors - Amr R Ghanam
	Pharmaceuticals (Basel); GRK2 Mediates Macrophage Polarization by Regulating EP4-cAMP-pCREB Signaling in Ulcerative Colitis and the Therapeutic Effect of Paroxetine on Mice with DSS-Induced Colitis; Authors - Jiawei Zhang
IF	Biol Trace Elem Res; ERK/PKM2 Is Mediated in the Warburg Effect and Cell Proliferation in Arsenic-Induced Human L-02 Hepatocytes; Authors - Fanshuo Yin
IF	Mol Neurobiol; Transcription Factor BCL11A Regulates Schwann Cell Behavior During Peripheral Nerve Regeneration; Authors - Yunsong Zhang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Baptiste (Verified Customer) (06-30-2023)

Good signal at 1/1000, low background.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse brain slice