ChromoTek Nano-Secondary® anti-mouse IgG1, recombinant VHH, CoraLite® Plus 647 [CTK0103, CTK0104]

Validation Data Gallery

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  Immunofluorescence analysis of HeLa cells stained with mouse IgG1 anti-CD147 antibody and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 647 (smsG1CL647-1, magenta). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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  Immunofluorescence analysis of HeLa cells co-stained with mouse IgG2b anti-tubulin beta antibody and mouse IgG1 anti-CD147 antibody followed by Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 488 (smsG2bCL488-1, green) and Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 647 (smsG1CL647-1, magenta). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

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  HEK-293 cell lysates were subjected to SDS-PAGE followed by multiplex western blot analysis with 3 mouse primary antibodies including anti-Lamin B1 (66095-1-Ig), anti-HNRNPA (67445-1-Ig), and anti-Tom20 (66777-1-Ig). Primary antibodies were detected using 3 mouse IgG subclass-specific nano-secondary reagents including Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, CoraLite® Plus 647 (smsG1CL647-1, blue), Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 750 (smsG2aCL750-1, white), and Nano-Secondary® alpaca anti-mouse IgG2b, recombinant VHH, CoraLite® Plus 488 (smsG2bCL488-1, green).

Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

Cat No. smsG1CL647-1

Publications(1)

Applications

IF, WB

Reactivity

mouse IgG1

Host

Alpaca

Conjugate

CoraLite® Plus 647

Synonyms

Alpaca single domain antibody, VHH, Nanobody, binding domain of single domain antibody, Nano-antibody

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Product Information

Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH is an anti-mouse IgG subclass specific antibody. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
• Applications: IF, WB
• Host: Alpaca-derived, recombinantly produced in bacteria
• Species Reactivity: Mouse IgG1 Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3, IgM
• Target/Specificity: Fc-fragment of mouse IgG1
• Physical State: Liquid
• Conjugate: CoraLite® Plus 647
• Suggested Dilution: Immunofluorescence 1:500; Western blot 1: 500
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Nanobody
• Class: Recombinant
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG2a, IgG2b, IgG2c, IgG3, and IgM
• Immunogen: Purified mouse IgG1
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Clones: CTK0103 (VHH0302), CTK104 (VHH0305)
• RRID: AB_2941312
• Affinity: CTK0103: KD =0.13 nM, CTK0104: KD = 0.63nM
• Excitation / Emission: Excitation max: 654 nm, Emission max: 674 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Shipped at ambient temperature. Store at +4°C short term or -20°C long term. Stable for 1 year at -20°C.
• Size: 10 μL; 100 μL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Mol Neurobiol; Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay; Authors - Yifen Shen