ChromoTek Nano-Secondary® anti-mouse IgG1, recombinant VHH, Alexa Fluor® 568 [CTK0103, CTK0104]

Validation Data Gallery

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  Anti-mouse IgG1 Nano-Secondary: Well-defined and characterized immunostaining. Primary anti-mouse IgG1 antibody (grey) with 2X2 monoclonal mouse Fc- specific Nanobodies (green) bound. In total, 8 fluorophores (red stars) label the mouse IgG1 primary antibody.

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  One-step immunostaining is the simultaneous incubation of mouse IgG1 primary antibody and anti-mouse IgG1 Nano-Secondary. This method reduces incubation and hands-on time. Simultaneous incubation also supports multiplexing, tissue penetration, and cell staining for flow cytometry.

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  HeLa cells were immunostained with mouse IgG1 anti-αTubulin antibody + alpaca anti-mouse IgG1 VHH Alexa Fluor® 568 (red). Nuclei were stained with DAPI (blue). Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

Cat No. sms1AF568-1

Publications(8)

Applications

IF, WB, FC

Conjugate

Alexa Fluor® 568

Host

Alpaca

Reactivity

Mouse IgG1

Synonyms

mouse IgG1; mouse immunoglobulin Gamma 1

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Product Information

Nano-Secondary® anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.

• Description: Nano-Secondary® alpaca anti-mouse IgG1, Fc-specific recombinant VHH reagent uses a novel class of anti-mouse IgG isotype specific antibodies. This secondary antibody product consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity. Nano-Secondary reagents enable simultaneous incubation with primary antibodies, thus shortening your experimental. Due to their small size, Nano-Secondary reagents provide enhanced image resolution. No cross-reactivity against serum proteins from other species, details see below.
• Applications: IF, WB, FC
• Host: Alpaca
• Species Reactivity: Mouse IgG1 Fc-fragment; No cross-reactivity: goat, guinea pig, rabbit, rat, sheep, human, macaque serum proteins, mouse IgG2a, IgG2b, IgG2c, IgG3
• Target / Specificity: Fc-fragment of mouse IgG1
• Physical State: Liquid
• Conjugate: Alexa Fluor® 568
• Suggested Dilution: Immunofluorescence 1:500; Super-resolution microscopy 1:500; Western blot 1:1,000
• Purification Method: Recombinant expression, affinity purification IMAC
• Type: Mixture of 2 monoclonal Nanobodies
• Class: Recombinant
• Format: Alpaca single domain antibody, monovalent
• Cross-reactivity: No cross-reactivity to goat, guinea pig, human, macaque, rabbit, rat, and sheep serum and to mouse IgG2a, IgG2b, IgG2c, and IgG3
• Immunogen: Purified mouse IgG1
• Clonality: Biclonal: mixture of 2 monoclonal Nanobodies
• Clones: CTK0103, CTK0104 (VHH0302, VHH0305)
• RRID: AB_2827579
• Affinity (KD): CTK0103: K_D =0.13 nM, CTK0104: K_D = 0.63nM
• Excitation / Emission: Excitation max: 578 nm, Emission max: 603 nm
• Storage Buffer: 10 mM HEPES pH 7.0, 500 mM NaCl, 5 mM EDTA, Preservative: 0.09 % Sodium azide
• Storage Condition: Store at -20°C short term or -80°C long term. Aliquot upon delivery. Avoid freeze-thaw cycles.
• Size: 10 µL; 100 µL
• Note: This product is for research use only, not for diagnostic or therapeutic use

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Publications

Application	Title
IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So
IF	Biosens Bioelectron; 3D co-culture of macrophages and fibroblasts in a sessile drop array for unveiling the role of macrophages in skin wound-healing; Authors - Xiaoyan Lyu
	Cell Mol Life Sci; SHANK3 deficiency leads to myelin defects in the central and peripheral nervous system.; Authors - Mariagiovanna Malara
IF	Vet Microbiol; NDV inhibited IFN-β secretion through impeding CHCHD10-mediated mitochondrial fusion to promote viral proliferation; Authors - Xibing Yu
IF	Biochim Biophys Acta Mol Basis Dis; The regulatory role of the apelin/APJ axis in scarring: Identification of upstream and downstream mechanisms; Authors - Nian Shi
IF	Cell Biol Int; Fat mass and obesity-associated protein alleviates Aβ(1-40) induced retinal pigment epithelial cells degeneration via PKA/CREB signaling pathway; Authors - Yifan Hu