Speedy One-Step ELISA Kits

The Speedy Kit is Proteintech's one-step ELISA solution for rapid analyte detection. Made with antibody pairs containing capture antibodies with Proteintech's FcZero backbone, these antibodies have been engineered to silence the Fc-receptor binding site, eliminating non-specific binding with Fc receptors sometimes present in serum and plasma samples.

  • Results in 90 minutes or less
  • Reduced assay complexity with single-wash step protocols
  • Unparalleled precision and sensitivity
  • Unmatched cost savings
Diagram of the Speedy One-Step ELISA Kit structure showing the FcZero backbone capture antibody configuration
Speedy One-Step ELISA Kit structure

Rapid, Single-Wash Step Workflow

Speedy Kits enable analyte detection in 90 minutes or less — nearly 3x faster than a traditional ELISA. Simply add prepared samples, standards, and antibodies in a single incubation step, wash, develop, and read.

Workflow diagram showing the Speedy One-Step ELISA process from sample and standard addition through wash, development, and readout in 90 minutes or less.
Speedy One-Step ELISA workflow showing analyte detection in 90 minutes or less.

Unparalleled Sensitivity

Despite their reduced assay time and simplified workflow, Speedy Kits maintain excellent assay performance and deliver higher sensitivity than comparable kits from leading competitors.

Sensitivity comparison of selected Speedy One-Step ELISA Kit targets versus leading competitor kits.
Target Proteintech sensitivity Leading competitor sensitivity Proteintech fold better sensitivity
IL-23 2.3 pg/mL 15.6 pg/mL 7x
AFP 7.32 pg/mL 44.8 pg/mL 6x
IL-12 2.3 pg/mL 13.6 pg/mL 6x
TNF-alpha 1.1 pg/mL 4.32 pg/mL 4x
IFN-gamma 1.1 pg/mL 3.43 pg/mL 3x
IL-2 2.67 pg/mL 4.9 pg/mL 2x

Speedy Kit Offering

Browse our Speedy One-Step ELISA Kit range by target, catalog number, and assay sensitivity.