Speedy™ Human CD134/OX40 One-Step ELISA Kit
Cat no : SE50068
Synonyms
TNFRSF4, ACT35, ACT35 antigen, CD134, OX40
Validation Data Gallery
Product Information
| Product name | Speedy™ Human CD134/OX40 One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 10.9 pg/mL |
| Range | 46.88-3000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7293 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 98% | 92%-102% |
| Cell culture supernatant | 106% | 97%-114% |
| Cell lysate | 80% | 75%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 1,323.5 | 24.8 | 1.9 |
| 2 | 8 | 329.5 | 9.7 | 2.9 |
| 3 | 8 | 163.4 | 4.0 | 2.4 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 1,324.5 | 26.1 | 2.0 |
| 2 | 16 | 329.2 | 9.9 | 3.0 |
| 3 | 16 | 164.0 | 5.3 | 3.2 |
Background Information
OX40 (CD134) is a co-stimulatory receptor belonging to the TNF receptor family. It is expressed on activated CD4+ T cells and plays a crucial role in enhancing T cell proliferation, cytokine production, and survival. OX40 signaling leads to the activation of NF-κB and the upregulation of anti-apoptotic proteins such as Bcl-xL and Bcl-2. This receptor also promotes the differentiation of naive CD4+ T cells into high IL-4-producing effectors. OX40 ligand (OX40L) is expressed on antigen-presenting cells (APCs) such as activated B cells and dendritic cells. The interaction between OX40 and OX40L is critical for T cell activation and the regulation of immune responses.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TNFRSF4, ACT35, ACT35 antigen, CD134, OX40 |
FAQ
1、I have limited sample diluent. How should I handle samples requiring high dilution factors?
Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows:
Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample.
Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample.
Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample.
If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
2、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.