Speedy™ Human/Cynomolgus Monkey IFN-gamma One-Step ELISA Kit
Cat no : SE50034
Synonyms
IFNG, IFG, IFN gamma, IFN γ, IFNγ
Validation Data Gallery
Product Information
| Product name | Speedy™ Human/Cynomolgus Monkey IFN-gamma One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human serum, Human plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.1 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human/Cynomolgus Monkey |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3458 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 106% | 98%-113% |
| Cell culture supernatant | 102% | 96%-105% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 249.3 | 6.0 | 2.4 |
| 2 | 8 | 63.0 | 3.8 | 6.0 |
| 3 | 8 | 32.8 | 1.4 | 4.2 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 245.0 | 10.2 | 4.2 |
| 2 | 16 | 62.9 | 3.4 | 5.5 |
| 3 | 16 | 32.6 | 1.3 | 4.0 |
Background Information
Interferon gamma (IFNG) is a soluble cytokine that is the only member of the type II class of interferons. It is secreted by Th1 cells, cytotoxic T cells and NK cells. The cytokine is associated with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. It plays a crucial role in pathogen clearance. Aberrant IFNG expression is associated with a number of autoinflammatory and autoimmune diseases. It has been identified in many studies as a biomarker for pleural tuberculosis (TB). Mutations in this gene are associated with aplastic anemia.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | IFNG, IFG, IFN gamma, IFN γ, IFNγ |
FAQ
1、If thermostatic shaker is not available, will it affect the duration of my experiment and the experimental data?
Answer: If thermostatic shaker is not available, it may take 2.5 hours to complete this experiment. After adding the antibody cocktail, we suggest you incubate the plate at 37℃ for 2 hours. After adding TMB, you only need to incubate the plate at 37℃ for 15 minutes. Through comparative experiments, our R&D personnel have confirmed that you can still obtain valid experimental data, although the signal will be slightly reduced.