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Cas9: A versatile tool for gene engineering
Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (clustered regularly interspaced short palindromic repeats) system. CRISPR forms part of the adaptive immune system and can help to protect against different viruses.
|Cas9||Rabbit polyclonal||26758-1-AP||ELISA, WB|
Recombinant protein was subjected to SDS PAGE followed by western blotting with Cas9-specific antibody (26758-1-AP) at a dilution of 1:3000.
Remarkable progress in CRISPR technology has been made over recent years due to Cas9 and hundreds of recent publications focusing on this small RNA-guided DNA endonuclease enzyme. When Cas9 is specifically located within the genome, it has the ability to precisely edit the genome. Cas9 controls diverse cellular functions and behavior by co-localizing proteins, RNA, or DNA. Therefore, Cas9 and CRISP are used as a tool for synthetic biology.
The main components of the CRISPR system are:
The gRNA is a very short synthetic RNA that binds to Cas9 and defines the target of interest to be modified. The target can easily be changed by changing the gRNA. As the design of gRNAs is quite simple, CRISPR become a powerful tool in genome editing. Originally, CRISPR was only used for knock-out experiments. Recent modifications of Cas9 also opened the way for selective repression or activation of the target of interest or purification of a certain DNA region.
|Loading control antibodies|
|Catalog no.: 60004-1-Ig|
GAPDH is commonly used as a protein loading control in western blot due to its consistently high expression in most cell types. This enzyme participates in several cellular events such as glycolysis, DNA repair, and apoptosis.
Proteintech monoclonal GAPDH antibodies are raised against a whole-protein antigen of human origin and have over 2,670 citations.
|Beta Actin Antibody (KD/KO validated)
|Catalog no.: 66009-1-Ig|
Beta-actin is usually used as a loading control due to its broad and consistent expression across all eukaryotic cell types and the fact that expression levels of this protein are not affected by most experimental treatments.
66009-1-Ig has been cited over 1,135 publications and has wide species reactivity.
Proteintech control antibodies are just $99 each (€150 in Europe) for a 150ul size vial.
|Prestained Protein Ladder (free samples available)||Broad Range Prestained Protein Ladder|
|Molecular weight coverage: 10-180 kDa||Molecular weight coverage: 3-245 kDa|
|Catalog no.: PL00001
||Catalog no.: PL00002
|Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.||Resolution of the prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.|
Yu, X. et al. Biotechnol Lett. 2016 Jun; 38: 919–29.
Shin, S. et al. Sci Rep. 2016 Jun 13;6: 27810.
Lander, Es. et al. Cell. 2016 Jan 14; 164: 18–28.