You've been automatically redirected here from Humanzyme.com, which is now part of Proteintech Group. Click here to dismiss this alert.
Important Customer Notice Regarding Covid-19 Outbreak. Read more.

CAS9 / CRISPR Antibody

Cas9: A versatile tool for gene engineering

Cas9: A versatile tool for gene engineering


Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (clustered regularly interspaced short palindromic repeats) system. CRISPR forms part of the adaptive immune system and can help to protect against different viruses.

Antibody Type Catalog no. Applications
Cas9 Rabbit polyclonal 26758-1-AP ELISA, WB
View the Cas9 product page here
Cas9  
Recombinant protein was subjected to SDS PAGE followed by western blotting with Cas9-specific antibody (26758-1-AP) at a dilution of 1:3000

Recombinant protein was subjected to SDS PAGE followed by western blotting with Cas9-specific antibody (26758-1-AP) at a dilution of 1:3000.

Cas9 and CRISPR

Remarkable progress in CRISPR technology has been made over recent years due to Cas9 and hundreds of recent publications focusing on this small RNA-guided DNA endonuclease enzyme. When Cas9 is specifically located within the genome, it has the ability to precisely edit the genome. Cas9 controls diverse cellular functions and behavior by co-localizing proteins, RNA, or DNA. Therefore, Cas9  and CRISP  are used as a tool for synthetic biology.

CRISPR   mechanism

The main components of the CRISPR  system are:

  • Cas9
  • Guide RNA (gRNA)

The gRNA is a very short synthetic RNA that binds to Cas9 and defines the target of interest to be modified. The target can easily be changed by changing the gRNA. As the design of gRNAs is quite simple, CRISPR  become a powerful tool in genome editing. Originally, CRISPR  was only used for knock-out experiments. Recent modifications of Cas9 also opened the way for selective repression or activation of the target of interest or purification of a certain DNA region.

Related products
Protein Ladders
Prestained Protein Ladder (free samples available) Broad Range Prestained Protein Ladder 
Molecular weight coverage: 10-180 kDa  Molecular weight coverage: 3-245 kDa
Catalog no.: PL00001
Catalog no.: PL00002
prestained protein marker with 10 markers covering a wide range of molecular weights from 10 to 180 kDa Broad range protein marker
Resolution of the prestained protein ladder in a 10-20% Tris-glycine gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane. Resolution of the prestained protein ladder in a 4-12% Bis-Tris gel (SDS-PAGE). The image shows the migration pattern in the gel and after transfer to a PVDF membrane.

 

Recent news in Cas9 and CRISPR

Yu, X. et al. Biotechnol Lett. 2016 Jun; 38: 919–29.

Shin, S. et al. Sci Rep. 2016 Jun 13;6: 27810.

Lander, Es. et al. Cell. 2016 Jan 14; 164: 18–28. 

Featured Products

Posted:
31 March, 2020

Share: