• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-3 protein- GMP grade

EC50

0.4-2.0 ng/mL EC50

Specific Activity

8.0 x 10^5 IU/mg calibrated against IL-3 reference standard (NIBSC cat no: 91/510). Reference use only. Specific activity is not routinely tested

Species Reactivity

human

Purity

>95 %

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1074-GMP

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor



Technical Specifications

GeneID 3562
Species Human
Expression HEK293
EC50 0.4-2.0 ng/mL EC50
Specific Activity8.0 x 10^5 IU/mg calibrated against IL-3 reference standard (NIBSC cat no: 91/510). Reference use only. Specific activity is not routinely tested
Purity >95 %
Endotoxin< 0.1 EU/μg
Accession NumberP08700
Molecular Mass 17 to 40 kDa, monomer, glycosylated
Formulation 1xPBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor