HumanKine® recombinant human IL-7 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.1-1.4 ng/mL EC50

Specific Activity

>7.00x10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

3

Cat no : HZ-1281

Synonyms

IL 7, IL7, IL-7, interleukin 7

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Validation Data Gallery

• 

  Purity of recombinant human IL-7 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human IL-7 (HZ-1281) stimulates dose-dependent proliferation of murine 2E8 human cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

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  Proteintech IL-7 (HZ-1281) demonstrates 3-fold lower EC50 and equivalent induction of proliferation compared to a leading competitor. Recombinant human IL-7 stimulates dose-dependent proliferation of murine 2E8 human cell line. Cell number was quantitatively assessed by PrestoBlue® Cell Viability Reagent. 2E8 cells were treated with increasing concentrations of recombinant IL-7 for 120 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.1-1.4 ng/mL.

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Technical Specifications

• GeneID: 3574
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.1-1.4 ng/mL EC50
• Specific Activity: >7.00x10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P10168
• Molecular Mass: 19, 25, and 30 kDa, monomer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Interleukin-7 (IL7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration (PMID: 29663382; 29655570; 29449560)

Synonyms

IL 7, IL7, IL-7, interleukin 7

Publications

Species	Title
human	Front Oncol; Combination of 4-1BB and DAP10 promotes proliferation and persistence of NKG2D(bbz) CAR-T cells; Authors - Cheng Wei
	Biochem J; Proteomic analysis in primary T cells reveals IL-7 alters T cell receptor thresholding via CYTIP/cytohesin/LFA-1 localisation and activation; Authors - Rayner M L Queiroz
rat	Heliyon; Astragalus regulates the intestinal immune response during sepsis by mediating ILC3 proliferation through RORγt; Authors - Jin Li