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Animal-free Recombinant Human Wnt3A
Humankine®1 Publications

Catalog number: HZ-1296

HEK293 expressed Endotoxin-free Animal-component free

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Freight/Packing £00.00

Format: Lyophilized

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Related Recombinant Proteins

Technical Specifications

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Wnt3A activity assay

The activity was determined by dose-dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells using pNPP as chromogenic substrate and stabilization of beta catenin in mouse L929 cells

Wnt3A purity

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
89780
Species:
Human
Expression:
HEK293
Activity:
Typically ≤ 20 ng/mL EC50
Purity:
>90%
Molecular Mass:
38-42 kDa
Formulation
1x PBS pH 7.4 + 500mM NaCl +1% CHAPS, See Certificate of Analysis for details
Species Reactivity:
human, mouse

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Recombinant Human Wnt3A is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins. These proteins are associated with oncogenesis and in numerous developmental processes. These processes include regulation of cell fate and patterning during embryogenesis. Human Wnt3A is a protein which shows 84% identity relative to other human Wnt3 proteins. Wnt3A plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnts are essential for their efficient secretion and biological activity.



Background:

Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)



Synonyms:
Wnt3A



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