HumanKine® recombinant human Wnt3A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

2-17 ng/mL EC50

Species Reactivity

Human

Purity

>90%

Publications

2

Cat no : HZ-1296

Synonyms

Protein Wnt 3a, WNT3A

---

Validation Data Gallery

• 

  The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

• 

  Recombinant human Wnt3A (HZ-1296) induces dose-dependent alkaline phosphatase production in MC3T3 E1 mouse preosteoblast cells using pNPP as chromogenic substrate. MC3T3 E1 cells were treated with increasing concnetrations of recombinant Wnt3A for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 2-17 ng/mL.

• 

  Recombinant human Wnt3A (HZ-1296) induces dose-dependent luciferase production in a HEK293 TCF/LEF reporter cell line. Luciferase production was assessed by One-Step™ luciferase assay Kit. HEK293 TCF/LEF reporter cells were treated with increasing concentrations of recombinant Wnt3A for 6 hours. The EC50 was determined using a 4-Parameter non-linear regression model. The EC50 range is 25-125 ng/mL.

---

Technical Specifications

• GeneID: 89780
• Species: Human
• Expression: HEK293
• Activity: 2-17 ng/mL EC50
• Purity: >90%
• Endotoxin: <1 EU/μg
• Accession Number: P56704
• Molecular Mass: 38-42 kDa
• Formulation: 1x PBS pH 7.4 + 500mM NaCl +1% CHAPS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.
Reconstitution	Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1x PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Wnt3A is a member of the Wnt family that consists of structurally linked genes that encode secreted signalling proteins. This protein is associated with oncogenesis and also regulation of cell fate and patterning during embryogenesis. It plays a key role in maintaining the integrity of embryonic and adult tissues. The post-translational glycosylation and acylation of Wnt are essential for their efficient secretion and biological functions (PMID: 8244403; 11834740)

Synonyms

Protein Wnt 3a, WNT3A

Publications

Species	Title
human	Sci Signal; Intercellular transmission of the unfolded protein response promotes survival and drug resistance in cancer cells.; Authors - Jeffrey J Rodvold
human	Elife; Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures.; Authors - Konstantinos Gkatzis