Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GNL3 antibody (67169-1-lg) labeled with  FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-ATTO594​ (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of rat brain: FFPE rat brain sections were stained with anti-GFAP antibody (60190-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-650 (magenta).  Cell nuclei are in blue.

Immunofluorescence of mouse cerebellum: FFPE mouse cerebellum sections were stained with anti-GFAP antibody (60190-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-488 (green),  and anti-SOX10 antibody (66786-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-594 (red). Cell nuclei are stained with DAPI (blue).

Flow cytometry with PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD3 antibody (65133-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and PE-Streptavidin. Cells were co-stained with anti-human CD4 antibody (CL488-65143). Cells are not fixed, lymphocytes are gated. 

Flow cytometry with PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD3 antibody (65133-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and PE-Streptavidin. Cells were co-stained with anti-human CD4 antibody (CL488-65143). Cells are not fixed, lymphocytes are gated. 

Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD8 antibody (65135-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC. 1X10^6 human PBMC were stained with either mouse IgG2a isotype control or anti-human CD8 antibody (65135-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Immunohistochemical analysis of paraffin-embedded  human lung cancer tissue slide using anti-CK7 antibody (66483-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and used at a dilution of 1:800 (under 10x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded  human lung cancer tissue slide using anti-CK7 antibody (66483-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2a (KFA047) and used at a dilution of 1:800 (under 40x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin β antibody labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-ATTO594​ (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of human colon: FFPE human colon sections were stained with anti-KRT20 antibody (60183-1-Ig) labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-650 (magenta). Cell nuclei are in blue.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD4 antibody (65134-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD3 antibody (CL488-65151). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.

Flow cytometry of PBMC.  1X10^6 human PBMC were stained with either mouse IgG2b isotype control or anti-human CD20 antibody (65085-1-Ig), which are labeled with FlexAble Biotin Antibody Labeling Kit for Mouse IgG2b (KFA067) and Streptavidin-PE. Cells were co-stained with anti-human CD45 antibody (CL488-65109). Cells are not fixed, lymphocytes are gated.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-GM130 (11308-1-AP) labeled with FlexAble Biotin Kit for Rabbit IgG (KFA007) and Streptavidin-ATTO594​ (yellow). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 antibody (12987-1-AP) followed by polyclonal goat anti-rabbit AF488 secondary antibody (green), anti-GM130 antibody (11308-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit (KFA007) and Streptavidin-ATTO594​ (yellow), and anti-Tom20 antibody (11802-1-AP) separately labeled with FlexAble Biotin Antibody Labeling Kit (KFA007) and Streptavidin-AQ650 (red). Nuclei are stained with DAPI (blue). Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of mouse testis: FFPE mouse testis sections were stained with anti-TNP1 antibody (17178-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-488 (green), and anti-BOLL antibody (13720-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-594 (red).  Cell nuclei are stained with DAPI (blue). 

Flow cytometry of PBMC.  1X10^6 HEK293 cells were fixed with FoxP3/Transcription Factor Staining Buffer Kit (PF00011), then stained with anti-human Mitofilin antibody (10179-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-APC. Single cells are gated.

Flow cytometry of PBMC.  1X10^6 HEK293 cells were fixed with FoxP3/Transcription Factor Staining Buffer Kit (PF00011), then stained with anti-human Mitofilin antibody (10179-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and Streptavidin-PE. Single cells are gated.

Immunohistochemical analysis of paraffin-embedded Rabbit colon tissue using anti-Cytokeratin 20 antibody (17329-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and used at a dilution of 1:1000 (under 10x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded Rabbit colon tissue using anti-Cytokeratin 20 antibody (17329-1-AP) labeled with FlexAble Biotin Antibody Labeling Kit for Rabbit IgG (KFA007) and used at a dilution of 1:1000 (under 40x lens). Streptavidin Poly-HRP and DAB substrate was used for detection. Heat mediated antigen retrieval performed with Tris-EDTA buffer (pH 9.0).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD45 antibody (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD4 antibody (clone RPA-T4, 65143-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA026).

FlexAble Workflow

PFA-fixed HeLa cells were stained with anti-GNL3 (67169-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and anti-Tubulin (66240-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA044, grey). Epifluorescence images were acquired with a 20x objective and post-processed.

PFA-fixed HeLa cells were stained with anti-Actin labeled with FlexAble CoraLite® Plus 405 Kit (KFA046, cyan) and Sytox Deep Red (cell nuclei, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD45 (clone HI30, 65109-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD3 (clone OKT3, 65133-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA024) and anti-human CD8 (clone UCHT4, 65204-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA046).

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA066, cyan) and anti-HDAC2 (67165-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit (KFA064, red). Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with anti-human CD3 (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite Plus 555 Kit (KFA022) and anti-human CD4 (clone OKT4, 65134-1-Ig) labeled with FlexAble CoraLite® Plus 405 Kit (KFA066).  Cells are not fixed, lymphocytes are gated. 

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TOM70 (14528-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan). Cell nuclei are in red. Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Flow cytometry of PBMC. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, cyan) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red).

Flow cytometry of HEK293. 1X10^6 HEK293 cells were intracellularly stained with 0.5 µg anti-human P62 (SQSTM1) antibody (18420-1-AP) labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, blue) or with 0.5 µg isotype control antibody labeled with FlexAble CoraLite® Plus 405 Kit (KFA006, red) or with FlexLinker CoraLite® Plus 405 for rabbit IgG (orange).  Unstained HEK293 cells were used as a reference (red). 

FlexAble Workflow

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green), anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, red) and anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, cyan).​ Confocal images were acquired with a 100x oil objective and post-processed. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Lamin B1 (66095-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-HSP60 (66041-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit (KFA022, blue), anti-GORASP2 (66627-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, yellow) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 750 Kit (KFA024, grey).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Ki67 labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 647 Kit (KFA023, magenta). Epifluorescence images were acquired with a 20x objective and post-processed.

Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-Tubulin alpha labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green) and DAPI (blue).​ Epifluorescence images were acquired with a 20x objective and post-processed.

Flow cytometry of PBMCs. 1X10^6 human peripheral blood mononuclear cells (PBMCs) were stained with 0.5 µg anti-human CD3 antibody (clone UCHT1, 65151-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021).

WB of HEK-293 cell lysates: HEK-293 cell lysates were detected with anti-GAPDH (10494-1-AP) labeled with FlexAble CoraLite® Plus 750 Kit (KFA004, red) and anti-NUDT21 (66335-1-Ig) labeled with FlexAble CoraLite® Plus 488 Kit (KFA021, green).

FlexAble Workflow