Tested Applications
| Positive WB detected in | HL-60 cells, THP-1 cells, human placenta tissue |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83794-4-RR targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag19503 Product name: Recombinant human ALOX5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC130332 Sequence: MPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQSSWNDFADFEKIFVKISNTISERVMNHWQEDLMFGYQFLNGCNPVLIRRCTELPEKLPVTTEMVECSLERQLSLEQEVQQGNIFIVDFELLDGIDANKTDPCTLQFLAAPICLLYKNLANKIVPIAIQLNQIPGDENPIFLPSDAKYDWL Predict reactive species |
| Full Name | arachidonate 5-lipoxygenase |
| Calculated Molecular Weight | 674 aa, 78 kDa |
| Observed Molecular Weight | 70-78 kDa |
| GenBank Accession Number | BC130332 |
| Gene Symbol | 5 Lipoxygenase |
| Gene ID (NCBI) | 240 |
| RRID | AB_3671385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P09917 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for 5 Lipoxygenase antibody 83794-4-RR | Download protocol |
| WB protocol for 5 Lipoxygenase antibody 83794-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The 5-Lipoxygenase Rabbit Recombinant Monoclonal Antibody (Cat# 83794-4-RR) has 1 citation to date. It appears in Oncology Letters (2026), where it was used in Western Blot to study how overexpression and knockdown of 5-lipoxygenase regulates migration and invasion of colorectal cancer cells. The research field is colorectal cancer biology, specifically cell migration and invasion mechanisms.
| Species | Application | Title |
|---|---|---|
Oncol Lett Overexpression and knockdown of 5-lipoxygenase regulates the migration and invasion of colorectal cancer cells.
|
Reviews
What customers say
One reviewer rated this product 2 out of 5 stars. They used it on SK-N-BE(2) cells in cell culture at a 1:1000 dilution and reported observing multiple bands, suggesting the antibody may lack specificity under their conditions. No other reviews are currently available for this catalog number.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nazek (Verified Customer) (07-23-2025) | saw multiple bands
 |
