A2BP1 Polyclonal antibody
A2BP1 Polyclonal Antibody for IF, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, RIP, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22647-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
RIP | See 1 publications below |
Product Information
The immunogen of 22647-1-AP is A2BP1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | A2BP1 fusion protein Ag18527 |
Full Name | ataxin 2-binding protein 1 |
Calculated molecular weight | 395 aa, 42 kDa |
Observed molecular weight | 45-50 kDa |
GenBank accession number | BC113691 |
Gene symbol | A2BP1 |
Gene ID (NCBI) | 54715 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.
Protocols
Product Specific Protocols | |
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IHC protocol for A2BP1 antibody 22647-1-AP | Download protocol |
IF protocol for A2BP1 antibody 22647-1-AP | Download protocol |
IP protocol for A2BP1 antibody 22647-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Ther Oncolytics RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.
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