AADACL1 Antibody

AADACL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14021-1-AP

Synonyms

AADACL1, KIAA1363, KIAA1363, NCEH1,NCEH, NCEH, NCEH1



Tested Applications

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14021-1-AP targets AADACL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AADACL1 fusion protein Ag5136
Full Name arylacetamide deacetylase-like 1
Calculated molecular weight 46 kDa
Observed molecular weight 55 kDa
GenBank accession number BC047588
Gene symbol AADACL1
Gene ID (NCBI) 57552
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AADACL1(Arylacetamide deacetylase-like 1) is also named as NCEH1, KIAA1363 and belongs to the 'GDXG' lipolytic enzyme family. The transmembrane enzyme, AADACL1, controls the production of the monoalkylglycerol ether (MAGE) class of NELs in cancer cells and acts as a 2-acetyl MAGE hydrolase and is likely the principal source for this activity in tumor cells(PMID:21513884). The full length protein has three glycosylation sites and can be N-glycosylated(PMID:19159218). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AADACL1 antibody 14021-1-APDownload protocol
IHC protocol for AADACL1 antibody 14021-1-APDownload protocol
IF protocol for AADACL1 antibody 14021-1-APDownload protocol
IP protocol for AADACL1 antibody 14021-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols