Tested Applications
| Positive WB detected in | human brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11244-1-AP targets AASDHPPT in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1763 Product name: Recombinant human AASDHPPT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-309 aa of BC016728 Sequence: MVFPAKRFCLVPSMEGVRWAFSCGTWLPSRAEWLLAVRSIQPEEKERIGQFVFARDAKAAMAGRLMIRKLVAEKLNIPWNHIRLQRTAKGKPVLAKDSSNPYPNFNFNISHQGDYAVLAAEPELQVGIDIMKTSFPGRGSIPEFFHIMKRKFTNKEWETIRSFKDEWTQLDMFYRNWALKESFIKAIGVGLGFELQRLEFDLSPLNLDIGQVYKETRLFLDGEEEKEWAFEESKIDEHHFVAVALRKPDGSRHQDVPSQDDSKPTQRQFTILNFNDLMSSAVPMTPEDPSFWDCFCFTEEIPIRNGTKS Predict reactive species |
| Full Name | aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC016728 |
| Gene Symbol | AASDHPPT |
| Gene ID (NCBI) | 60496 |
| RRID | AB_2304862 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRN7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AASDHPPT, also known as L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase, is required for mitochondrial respiration and oxidative metabolism via the mitochondrial fatty acid synthesis (mtFAS) pathway. AASDHPPT catalyzes the post-translational modification of target proteins by phosphopantetheine and transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, including the acyl carrier domain of FASN.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AASDHPPT antibody 11244-1-AP | Download protocol |
| WB protocol for AASDHPPT antibody 11244-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AASDHPPT polyclonal antibody (Cat# 11244-1-AP) has 2 total citations. These appear in Metabolism (IF 16.7, 2025) and bioRxiv (2024), both by Pieter R Norden et al. The research focuses on mitochondrial phosphopantetheinylation and its role in oxidative metabolism and function, specifically involving the mitochondrial fatty acid synthesis (mtFAS) pathway in mouse models. Applications cited include Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Metabolism Mitochondrial phosphopantetheinylation is required for oxidative metabolism
| ||
