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Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cellls |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
14628-1-AP targets ABCB10 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABCB10 fusion protein Ag6203 Predict reactive species |
| Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 10 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC064930 |
| Gene Symbol | ABCB10 |
| Gene ID (NCBI) | 23456 |
| RRID | AB_2220668 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRK6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCB10 antibody 14628-1-AP | Download protocol |
| IHC protocol for ABCB10 antibody 14628-1-AP | Download protocol |
| IP protocol for ABCB10 antibody 14628-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun ABCB10 depletion reduces unfolded protein response in mitochondria.
| ||
Exp Hematol Abcb10 regulates murine hematopoietic stem cell potential and erythroid differentiation | ||
Mol Cell Unraveling ETC complex I function in ferroptosis reveals a potential ferroptosis-inducing therapeutic strategy for LKB1-deficient cancers |
