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ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51007-1-AP

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ABCB6 Polyclonal antibody (51007-1-AP)

    WB analysis using 51007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51007-1-AP (ABCB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ABCB6 Polyclonal antibody (51007-1-AP)

    IF Staining of MCF-7 using 51007-1-AP

    Immunofluorescent analysis of MCF-7 cells using 51007-1-AP (ABCB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, U-251 cells
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

51007-1-AP targets ABCB6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCB6 fusion protein Ag0461
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 6
Calculated Molecular Weight 94 kDa
Observed Molecular Weight70-79 kDa
GenBank Accession NumberBC000559
Gene Symbol ABCB6
Gene ID (NCBI) 10058
RRIDAB_2220830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCB6 antibody 51007-1-APDownload protocol
IF protocol for ABCB6 antibody 51007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Invest Dermatol

Mutations in ABCB6 Cause Dyschromatosis Universalis Hereditaria.

Authors - Zhang Caie C
View Article
humanIHC

Cell Commun Signal

The ferroptosis and iron-metabolism signature robustly predicts clinical diagnosis, prognosis and immune microenvironment for hepatocellular carcinoma.

Authors - Bufu Tang
View Article
humanWB

PLoS One

Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells.

Authors - Takafumi Nakano
View Article
humanWB

Free Radic Biol Med

Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma

Authors - Zhao Yin
View Article