Tested Applications
| Positive WB detected in | mouse liver tissue, mouse thymus tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
11158-1-AP targets ABCB7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1544 Product name: Recombinant human ABCB7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 25-173 aa of BC006323 Sequence: AILIRPLVSVSGSGPQWRPHQLGALGTARAYQQIPESLKSITWQRLGKGNSGQFLDAAKALQVWPLIEKRTCWHGHAGGGLHTDPKEGLKDVDTRKIIKAMLSYVWPKDRPDLRARVAISLGFLGGAKAMNIVVPFMFKYAVDSLNQMS Predict reactive species |
| Full Name | ATP-binding cassette, sub-family B (MDR/TAP), member 7 |
| Calculated Molecular Weight | 753 aa, 83 kDa |
| Observed Molecular Weight | 83 kDa |
| GenBank Accession Number | BC006323 |
| Gene Symbol | ABCB7 |
| Gene ID (NCBI) | 22 |
| RRID | AB_2220945 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75027 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABCB7 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCB7 exports glutathione-coordinated iron-sulfur clusters from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur-containing proteins and participates in iron homeostasis(PMID: 33157103, 17192393). ABCB7 structure and mutation causing X- linked sideroblastic anemia and ataxia (XLSA/A) with disruption of cytosolic iron-sulfur protein maturation (PMID: 10196363,11050011).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ABCB7 antibody 11158-1-AP | Download protocol |
| WB protocol for ABCB7 antibody 11158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8. | ||
Arch Biochem Biophys Knockdown of ABCB7 inhibits esophageal cancer progression by inhibiting the TGF-β/Smad signaling
| ||
Mol Cell Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis |
