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WB analysis of BGC-823 using 11066-1-AP
BGC-823 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11066-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 11066-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 11066-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 11066-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse thymus using 11066-1-AP
IP result of anti-ABCB9 (IP:11066-1-AP, 4ug; Detection:11066-1-AP 1:500) with mouse thymus tissue lysate 4000 ug.
IHC staining of mouse testis using 11066-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11066-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11066-1-AP
Immunofluorescent analysis of HepG2 cells, using ABCB9 antibody 11066-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

ABCB9 Polyclonal antibody

Name: ABCB9 Polyclonal antibody
Brand: Proteintech
SKU: 11066-1-AP

ABCB9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11066-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TAPL, EC:7.4.2.6, ATP-binding cassette transporter 9, ATP-binding cassette sub-family B member 9, ABC-type oligopeptide transporter ABCB9

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	BGC-823 cells, HL-60 cells, human testis tissue, K-562 cells, mouse thymus tissue
Positive IP detected in	mouse thymus tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

11066-1-AP targets ABCB9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1542 Product name: Recombinant human ABCB9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-181 aa of BC017348 Sequence: EDIRHFNIFDSVLDLWAACLYRSCLLLGATIGVAKNSALGPRRLRASWLVITLVCLFVGIYAMVKLLLFSEVRRPIRDPWFWALFVWTYISLGASFLLWWLLSTVRPGTQALEPGAATEAEGFPGSGRPPPEQASGATLQKLLSYT Predict reactive species
Full Name	ATP-binding cassette, sub-family B (MDR/TAP), member 9
Calculated Molecular Weight	84 kDa
Observed Molecular Weight	72 kDa
GenBank Accession Number	BC017348
Gene Symbol	ABCB9
Gene ID (NCBI)	23457
RRID	AB_10898357
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NP78
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ABCB9 antibody 11066-1-AP	Download protocol
IHC protocol for ABCB9 antibody 11066-1-AP	Download protocol
IP protocol for ABCB9 antibody 11066-1-AP	Download protocol
WB protocol for ABCB9 antibody 11066-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncogene Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis Authors - Jing Ma View Article