|Positive WB detected in||HEK-293 cells, HeLa cells, NCI-H1299 cells, Y79 cells|
|Positive IHC detected in||human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue, HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15129-1-AP targets ABLIM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ABLIM1 fusion protein Ag7258|
|Full Name||actin binding LIM protein 1|
|Calculated molecular weight||88 kDa|
|Observed molecular weight||48-52 kDa, 105-110 kDa|
|GenBank accession number||BC002448|
|Gene ID (NCBI)||3983|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joleen (Verified Customer) (05-29-2019)
ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).