Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, NCI-H1299 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse skeletal muscle tissue |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IF | See 2 publications below |
Product Information
15129-1-AP targets ABLIM1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ABLIM1 fusion protein Ag7258 Predict reactive species |
| Full Name | actin binding LIM protein 1 |
| Calculated Molecular Weight | 88 kDa |
| Observed Molecular Weight | 48-52 kDa, 105-110 kDa |
| GenBank Accession Number | BC002448 |
| Gene Symbol | ABLIM1 |
| Gene ID (NCBI) | 3983 |
| RRID | AB_2257767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14639 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Due to the alternative splicing, ABLIM1 has multiple isoforms with predicted masses of 52, 70, and 88 kDa, respectively(PMID: 9245787). The phosphorylation or binding with SUMO2 may result in higher MW.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| IHC protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| IF protocol for ABLIM1 antibody 15129-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.
| ||
J Cell Physiol Actin binding LIM 1 (abLIM1) negatively controls osteoclastogenesis by regulating cell migration and fusion.
| ||
Int J Biol Sci Rictor promotes cell migration and actin polymerization through regulating ABLIM1 phosphorylation in Hepatocellular Carcinoma.
| ||
Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions. | ||
medRxiv Antigenic responses are hallmarks of fibrotic interstitial lung diseases independent of underlying etiologies | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Joleen (Verified Customer) (05-29-2019) | ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).
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