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ABLIM1 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 15129-1-AP

Featured Product KD/KO validated

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ABLIM1 Antibody WB HeLa cells 15129-1-AP

WB result of ABLIM1 antibody (15129-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABLIM1 transfected HeLa cells

ABLIM1 Antibody IF mouse skeletal muscle tissue 15129-1-AP

Immunofluorescent analysis of ( 4% PFA) fixed mouse skeletal muscle tissue using 15129-1-AP (ABLIM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

ABLIM1 Antibody WB HEK-293 cells 15129-1-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 15129-1-AP(ABLIM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

ABLIM1 Antibody IHC human liver tissue 15129-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP(ABLIM1 antibody) at dilution of 1:100 (under 10x lens)

ABLIM1 Antibody IHC human liver tissue 15129-1-AP

Immunohistochemical analysis of paraffin-embedded human liver using 15129-1-AP(ABLIM1 antibody) at dilution of 1:100 (under 40x lens)

Species specificity:
human, mouse, rat
Positive WB detected in:
HEK-293 cells, HeLa cells, NCI-H1299 cells, Y79 cells
Positive IHC detected in:
human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
mouse skeletal muscle tissue, HEK-293 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:20-1:200
IF : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ABLIM1 fusion protein Ag7258
Full name:
actin binding LIM protein 1
Calculated molecular weight:
88 kDa
Observed molecular weight:
48-52 kDa,105-110 kDa
GenBank accession number:
BC002448
Gene ID (NCBI):
3983
Gene symbol
ABLIM1
Synonyms
ABLIM, abLIM 1, ABLIM1, actin binding LIM protein 1, KIAA0059, LIMAB1, LIMATIN

Background

ABLIM1 (actin-binding LIM protein 1) is a member of the LIM-domain protein family, mediating interactions between actin filaments and cytoplasmic targets. ABLIM1 has been reported as a cell cortex protein specifically in non-erythrocytes, and is critical for the formation of dense interwoven cortical actin meshwork. Several isoforms of ABLIM1 exist due to the alternative splicing. This antibody recognized 110 kDa and 48 kDa forms of ABLIM1. The phosphorylation or binding with SUMO2 may result in higher MW (predicted MW 88 kDa).

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

ABLIM1 Rabbit Polyclonal antibody

Joleen (Verified Customer), 05-29-2019
Applications
Western Blot,
Primary Antibody Dilution
1:1000
ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)

ABLIM1 Antibody Western Blot, validation (1:1000 dilution) in MDCK GII (Cat no:15129-1-AP)

Cell/Tissue Type
MDCK GII
Lot Number
ABLIM should be around 87kD, but the 2 prominent bands do not come near that molecular weight. The top band is just above the 100kD molecular weight marker and the lower band is below the 75kD molecular weight marker. (BioRad Precision Plus Protein Standard used).
Proteintech Response
Thank you for alerting us to this issue. We will be in touch and will re-evaluate this antibody in QC.

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