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ACAP2 Polyclonal antibody

ACAP2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 14029-1-AP

Synonyms

ACAP2, Centaurin beta 2, CENTB2, CNT B2, KIAA0041

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using ACAP2 Polyclonal antibody (14029-1-AP)

    WB analysis of NIH/3T3 using 14029-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ACAP2 Polyclonal antibody (14029-1-AP)

    WB analysis of Jurkat using 14029-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ACAP2 Polyclonal antibody (14029-1-AP)

    WB analysis of Jurkat using 14029-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using ACAP2 Polyclonal antibody (14029-1-AP)

    IP experiment of NIH/3T3 using 14029-1-AP

    IP result of anti-ACAP2 (IP:14029-1-AP, 4ug; Detection:14029-1-AP 1:300) with NIH/3T3 cells lysate 3600ug.

Tested Applications

Positive WB detected inNIH/3T3 cells, Jurkat cells
Positive IP detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14029-1-AP targets ACAP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACAP2 fusion protein Ag5190
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
Calculated Molecular Weight 778 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC060767
Gene Symbol ACAP2
Gene ID (NCBI) 23527
RRIDAB_2219956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACAP2 antibody 14029-1-APDownload protocol
IP protocol for ACAP2 antibody 14029-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB,IF

J Biol Chem

Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis.

Authors - James W Clancy
View Article
mouseWB

Cell Death Dis

Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.

Authors - Viktoriya Zhuravleva
  • KD Validated
View Article