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Tested Applications
| Positive WB detected in | NIH/3T3 cells, Jurkat cells |
| Positive IP detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
14029-1-AP targets ACAP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACAP2 fusion protein Ag5190 Predict reactive species |
| Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 2 |
| Calculated Molecular Weight | 778 aa, 88 kDa |
| Observed Molecular Weight | 88 kDa |
| GenBank Accession Number | BC060767 |
| Gene Symbol | ACAP2 |
| Gene ID (NCBI) | 23527 |
| RRID | AB_2219956 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15057 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACAP2 antibody 14029-1-AP | Download protocol |
| IP protocol for ACAP2 antibody 14029-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis. | ||
Cell Death Dis Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.
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