Aconitase 2 Polyclonal antibody

Aconitase 2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11134-1-AP

Synonyms

ACO2, Aconitase, aconitase 2, aconitase 2, mitochondrial, ACONM, Citrate hydro lyase



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, HeLa cells, human heart tissue, human liver tissue, mouse heart tissue
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

11134-1-AP targets Aconitase 2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Aconitase 2 fusion protein Ag1590
Full Name aconitase 2, mitochondrial
Calculated molecular weight 85 kDa
Observed molecular weight 85 kDa
GenBank accession numberBC014092
Gene symbol ACO2
Gene ID (NCBI) 50
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACO2(aconitate hydratase, mitochondrial) is also named as citrate hydro-lyase and belongs to the aconitase/IPM isomerase family. It plays a key function in cellular energy production, and loss of its activity has a major impact on cellular and organismal survival. Western blot shows two bands of 83 kDa and 40 kDa. The 40 kDa fragment decreases with age and oxidative stress, whereas other fragmentation products with molecular weights between 40 and 83 kDa increased with age and MnSOD(mitochondrial manganese superoxide dismutase) deficiency(PMID:12459471). Defects in ACO2 are the cause of infantile cerebellar-retinal degeneration (ICRD).

Protocols

Product Specific Protocols
WB protocol for Aconitase 2 antibody 11134-1-APDownload protocol
IHC protocol for Aconitase 2 antibody 11134-1-APDownload protocol
IF protocol for Aconitase 2 antibody 11134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

miR-450a acts as a tumor suppressor in ovarian cancer by regulating energy metabolism.

Authors - Bruna Rodrigues Muys
mouseWB

Exp Neurobiol

Tumor Necrosis Factor-Associated Protein 1 (TRAP1) is Released from the Mitochondria Following 6-hydroxydopamine Treatment.

Authors - Dong-Ik Shin
humanWB

Toxicon

Ameliorative effect of ursolic acid on ochratoxin A-induced renal cytotoxicity mediated by Lonp1/Aco2/Hsp75.

Authors - Chen Li
human,mouseWB

Int J Mol Sci

Altered Aconitase 2 Activity in Huntington's Disease Peripheral Blood Cells and Mouse Model Striatum.

Authors - Chiung-Mei Chen
ratWB

Sci Rep

Bioenergetic functions in subpopulations of heart mitochondria are preserved in a non-obese type 2 diabetes rat model (Goto-Kakizaki).

Authors - N Lai
mouseWB

Sci Rep

Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts.

Authors - Huihui Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Svitlana (Verified Customer) (03-19-2019)

SDS-PAGE: mitochondrial lysate 10 ug protein/well, 4-12% Bis-Tris. Transfer: Immobilon-FL transfer membranes O/N at 30V, 4C Blocking: SEA Block Blocking Buffer 1h at room temperature. Secondary Ab: IRDye 800CW Goat anti-Rabbit 1 h at room temperature. Lines on WB: 1. BioRad Precision Plus Protein standard 2. Lysate of mitochondrial fraction.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293t mitochondria
Aconitase 2 Antibody Western Blot, validation (1:1000 dilution) in HEK293t mitochondria (Cat no:11134-1-AP)