Tested Applications
| Positive WB detected in | HEK-293 cells, human brain tissue, human kidney tissue, human testis tissue, mouse kidney tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human kidney tissue, human skeletal muscle tissue, human heart tissue, human testis tissue, human liver tissue, human spleen tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15633-1-AP targets ACOT1/2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8093 Product name: Recombinant human ACOT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 222-483 aa of BC006335 Sequence: IVDMFGTGGGLLEYRASLLAGKGFAVMALAYYNYEDLPKTMETLHLEYFEEAMNYLLSHPEVKGPGVGLLGISKGGELCLSMASFLKGITAAVVINGSVANVGGTLRYKGETLPPVGVNRNRIKVTKDGYADIVDVLNSPLEGPDQKSFIPVERAESTFLFLVGQDDHNWKSEFYANEACKRLQAHGRRKPQIICYPETGHYIEPPYFPLCRASLHALVGSPIIWGGEPRAHVMAQVDAWKQLQTFFHKHLGGHEGTIPSKV Predict reactive species |
| Full Name | acyl-CoA thioesterase 2 |
| Calculated Molecular Weight | 483 aa, 53 kDa |
| Observed Molecular Weight | 46-53 kDa |
| GenBank Accession Number | BC006335 |
| Gene Symbol | ACOT2 |
| Gene ID (NCBI) | 10965 |
| RRID | AB_2221535 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49753 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Acyl-CoA thioesterase (Acot)2 localizes to the mitochondrial matrix and hydrolyses long-chain fatty acyl-CoA into free FA and CoASH. Acot2 is expressed in highly oxidative tissues and is poised to modulate mitochondrial FA oxidation (FAO) (PMID: 25114170). The structure of ACOT2 consists of two domains, N and C domains, and the active site of ACOT2 is located at the interface between the N and C domains (PMID: 19497300). ACOT2 shares 98% homology with ACOT1, and this antibody can detect both ACOT1 and ACOT2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ACOT1/2 antibody 15633-1-AP | Download protocol |
| IHC protocol for ACOT1/2 antibody 15633-1-AP | Download protocol |
| IP protocol for ACOT1/2 antibody 15633-1-AP | Download protocol |
| WB protocol for ACOT1/2 antibody 15633-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ACOT1/2 Polyclonal antibody (Cat# 15633-1-AP) has 12 total citations spanning journals including Redox Biology, Developmental Cell, EMBO Molecular Medicine, Biochemical Pharmacology, Hepatology Communications, Toxicological Sciences, Journal of Biological Chemistry, European Journal of Immunology, Journal of Pharmaceutical and Biomedical Analysis, Life Science Alliance, Biomedical Research International, and American Heart Journal Plus. Research fields cover lipid/fatty acid metabolism, mitochondrial biology, ferroptosis, muscle atrophy, liver cirrhosis, diabetic cardiomyopathy, ovarian follicle development, rheumatoid arthritis, and adipocyte differentiation.
| Species | Application | Title |
|---|---|---|
Redox Biol Txnrd2 loss in skeletal muscle causes muscle atrophy and drives leanness and obesity resistance. | ||
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy | ||
Biochem Pharmacol Transcriptome sequencing analysis reveals the molecular regulatory mechanism of myocardial hypertrophy induced by angiotensin II | ||
Hepatol Commun Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis | ||
Toxicol Sci Quantitative Label-Free Proteomic Analysis of Mouse Ovarian Antral Follicles Following Oral Exposure to a Human Relevant Mixture of Three Phthalates |
Reviews
What customers say
One reviewer (4/5 stars) used this antibody for Western Blot at 1:1000 on human and mouse brain tissues and rat PC12 cells. They observed a specific 48 kDa band in human samples only, but noted a strong non-specific band at 40 kDa across all sample types. The reviewer provided detailed protocol conditions including 15 µg of RIPA lysate on a 4–12% Bis-Tris gradient gel with overnight transfer and SEA Block blocking buffer.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lana (Verified Customer) (06-21-2019) | Lines on WB:1. BioRad Precision Plus Protein standards2. Homogenate of human cortex3. Non-synaptosomal mitochondria of human cortex4. Homogenate of mouse brains5. Non-synaptosomal mitochondria of mouse brains6. Whole cell lysate of rat PC12 cells7. Mitochondria of rat C12 cellsNotes: Specific band 48 kDa in human samples only. Strong non-specific band 40 kDa in all samples types.WB protocol:SDS-PAGE: 15 ug/ul of RIPA lysates, 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore), O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit, 1 h incubation at room temperature.
 |
