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ACRV1 Polyclonal antibody

ACRV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14040-1-AP

Synonyms

Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2

Validation Data Gallery

  • IHC staining of human testis using 14040-1-AP

    IHC staining of human testis using 14040-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 14040-1-AP

    IHC staining of human testis using 14040-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis of DU 145 using 14040-1-AP

    WB analysis of DU 145 using 14040-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP (ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of DU 145 using 14040-1-AP

    IP experiment of DU 145 using 14040-1-AP

    IP Result of anti-ACRV1 (IP:14040-1-AP, 3ug; Detection:14040-1-AP 1:2000) with DU 145 cells lysate 560ug.

  • IF Staining of PC-3 using 14040-1-AP

    IF Staining of PC-3 using 14040-1-AP

    Immunofluorescent analysis of PC-3 cells using 14040-1-AP (ACRV1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDU 145 cells, LNCaP cells, PC-3 cells
Positive IP detected inDU 145 cells
Positive IHC detected inhuman testis tissue,
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 2 publications below

Product Information

14040-1-AP targets ACRV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACRV1 fusion protein Ag5109
Full Name acrosomal vesicle protein 1
Calculated molecular weight 28 kDa
Observed molecular weight 13-36 kDa
GenBank accession numberBC014588
Gene symbol ACRV1
Gene ID (NCBI) 56
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACRV1 antibody 14040-1-APDownload protocol
IHC protocol for ACRV1 antibody 14040-1-APDownload protocol
IF protocol for ACRV1 antibody 14040-1-APDownload protocol
IP protocol for ACRV1 antibody 14040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acids Res

Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis.

Authors - Deqiang Ding
View Article
mouseIF

Nat Commun

TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice.

Authors - Deqiang Ding
View Article