ACRV1 Polyclonal antibody
ACRV1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 14040-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | DU 145 cells, LNCaP cells, PC-3 cells |
Positive IP detected in | DU 145 cells |
Positive IHC detected in | human testis tissue, Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | PC-3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
IF | See 2 publications below |
Product Information
14040-1-AP targets ACRV1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ACRV1 fusion protein Ag5109 |
Full Name | acrosomal vesicle protein 1 |
Calculated molecular weight | 28 kDa |
Observed molecular weight | 13-36 kDa |
GenBank accession number | BC014588 |
Gene symbol | ACRV1 |
Gene ID (NCBI) | 56 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ACRV1 antibody 14040-1-AP | Download protocol |
IHC protocol for ACRV1 antibody 14040-1-AP | Download protocol |
IF protocol for ACRV1 antibody 14040-1-AP | Download protocol |
IP protocol for ACRV1 antibody 14040-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nucleic Acids Res Mitochondrial membrane-based initial separation of MIWI and MILI functions during pachytene piRNA biogenesis. | ||
Nat Commun TDRD5 binds piRNA precursors and selectively enhances pachytene piRNA processing in mice. |