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ACRV1 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 14040-1-AP

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Con: 22 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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ACRV1 Antibody IHC human testis tissue 14040-1-AP

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 10x lens).

ACRV1 Antibody IHC human testis tissue 14040-1-AP

Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14040-1-AP (ACRV1 antibody) at dilution of 1:200 (under 40x lens).

ACRV1 Antibody WB DU 145 cells 14040-1-AP

DU 145 cells were subjected to SDS PAGE followed by western blot with 14040-1-AP(ACRV1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

ACRV1 Antibody IP DU 145 cells 14040-1-AP

IP Result of anti-ACRV1 (IP:14040-1-AP, 3ug; Detection:14040-1-AP 1:2000) with DU 145 cells lysate 560ug.

ACRV1 Antibody IF PC-3 cells 14040-1-AP

Immunofluorescent analysis of PC-3 cells using 14040-1-AP(ACRV1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
DU 145 cells, LNCaP cells, PC-3 cells
Positive IP detected in:
DU 145 cells
Positive IHC detected in:
human testis tissue,

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
PC-3 cells
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:500
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACRV1 fusion protein Ag5109
Full name:
acrosomal vesicle protein 1
Calculated molecular weight:
28 kDa
Observed molecular weight:
13-36 kDa
GenBank accession number:
BC014588
Gene ID (NCBI):
56
Gene symbol
ACRV1
Synonyms
Acrosomal protein SP 10, acrosomal vesicle protein 1, ACRV1, D11S4365, SP 10, SPACA2

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