• Featured Product
  • KD/KO Validated

ACSL1 Antibody

ACSL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat no : 13989-1-AP

Synonyms

ACS1, ACSL1, Acyl CoA synthetase 1, FACL1, FACL2, LACS, LACS 1, LACS 2, LACS1, LACS2, Palmitoyl CoA ligase 1, Palmitoyl CoA ligase 2



Tested Applications

Positive WB detected inL02 cells, HepG2 cells, human cerebellum tissue, mouse cerebellum tissue, rat liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, human heart tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13989-1-AP targets ACSL1 in IF, IHC, IP, WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL1 fusion protein Ag5059
Full Name acyl-CoA synthetase long-chain family member 1
Calculated molecular weight 78 kDa
Observed molecular weight 68 kDa,78 kDa
GenBank accession number BC050073
Gene symbol ACSL1
Gene ID (NCBI) 2180
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20oC. Aliquoting is unnecessary for -20oC storage.

Background Information

ACSL1(Long-chain-fatty-acid--CoA ligase 1) is also named as FACL1, FACL2, LACS, LACS1, LACS2 and belongs to the ATP-dependent AMP-binding enzyme family. ACSL1 is a 75 kDa protein that is associated peripherally with the plasma membrane(Brian M Wiczer, etc., 2006). ACSL1 is abundantly expressed in tissues, such as liver and brown fat, that metabolize substantial amounts of triglycerides as fuel, and as such, a deficiency in ACSL1 function could have a more profound affect in those cells, resulting in hepatosteatosis and potentially increased very low density lipoprotein production by the liver or decreased thermogenic capacity in brown adipose tissue(PMID:19429676). An anti-rat ACSL1 antibody recognized a band of the predicted 68 kDa in high-speed supernatant from rat liver and in human and murine SMCs, monocyte-derived macrophages, and murine peritoneal macrophages (PMID:17259370). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSL1 antibody 13989-1-APDownload protocol
IHC protocol for ACSL1 antibody 13989-1-APDownload protocol
IF protocol for ACSL1 antibody 13989-1-APDownload protocol
IP protocol for ACSL1 antibody 13989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
humanWB

Cell Chem Biol

Exogenous Monounsaturated Fatty Acids Promote a Ferroptosis-Resistant Cell State.

Authors - Leslie Magtanong
  • KO Validated
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
ratWB

Evid Based Complement Alternat Med

Chinese medicine formula lingguizhugan decoction improves Beta-oxidation and metabolism of Fatty Acid in high-fat-diet-induced rat model of Fatty liver disease.

Authors - Liu Tao T
humanWB,IHC

Biochem Biophys Res Commun

HBXIP up-regulates ACSL1 through activating transcriptional factor Sp1 in breast cancer.

Authors - Yue Wang
humanWB

Biochem Biophys Res Commun

Aspirin suppresses the abnormal lipid metabolism in liver cancer cells via disrupting an NFκB-ACSL1 signaling.

Authors - Guang Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Thomas Dupas (10-23-2019)

Very good service, very good antibody

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Rattus heart
No image submitted with review