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  • KD/KO Validated

ACSL4/FACL4 Polyclonal antibody

ACSL4/FACL4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22401-1-AP

Synonyms

ACS4, ACSL4, FACL4, LACS 4, LACS4, MRX63, MRX68



Tested Applications

Positive WB detected inCOLO 320 cells, HEK-293 cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22401-1-AP is ACSL4/FACL4 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL4/FACL4 fusion protein Ag18085
Full Name acyl-CoA synthetase long-chain family member 4
Calculated molecular weight 711 aa, 79 kDa
Observed molecular weight 79 kDa, 74 kDa
GenBank accession numberBC034959
Gene symbol ACSL4
Gene ID (NCBI) 2182
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IHC protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
IP protocol for ACSL4/FACL4 antibody 22401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
humanWB

Cell Death Dis

The oncometabolite 2-hydroxyglutarate produced by mutant IDH1 sensitizes cells to ferroptosis.

Authors - Tian-Xiang Wang
humanWB

Int J Clin Exp Pathol

Quantitative proteomic analysis identifies new effectors of FOXM1 involved in breast cancer cell migration.

Authors - Xiaojuan Ye
  • KD Validated
humanWB

Biochem Biophys Res Commun

Finvolvement of cholesterol in hepatitis B virus X protein-induced abnormal lipid metabolism of hepatoma cells via up-regulating miR-205-targeted ACSL4.

Authors - Ming Cui
humanWB,IHC

Mol Cell Biochem

The endogenous subcellular localisations of the long chain fatty acid-activating enzymes ACSL3 and ACSL4 in sarcoma and breast cancer cells.

Authors - Yassmeen Radif
humanIHC

Biosci Rep

Immunohistochemical staining reveals differential expression of ACSL3 and ACSL4 in hepatocellular carcinoma and hepatic gastrointestinal metastases.

Authors - Haarith Ndiaye