|Positive WB detected in||mouse brain tissue, HeLa cells, mouse liver tissue|
|Positive IP detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Sample-dependent, check data in validation data gallery|
15708-1-AP targets ACSL5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Reactivity||human, mouse, rat|
|Cited Species||mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACSL5 fusion protein Ag8258|
|Full Name||acyl-CoA synthetase long-chain family member 5|
|Calculated molecular weight||76 kDa|
|Observed molecular weight||66-70 kDa|
|GenBank accession number||BC007985|
|Gene ID (NCBI)||51703|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.
J Proteome Res
Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.
Potent effects of dioscin against obesity in mice.
Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.
J Biol Chem
The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.
Protective effects of dioscin against alcohol-induced liver injury.
Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.