ACSL5 Antibody

ACSL5 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15708-1-AP

Synonyms

ACS2, ACS5, ACSL5, FACL5, LACS 5



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse liver tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 7 publications below

Product Information

15708-1-AP targets ACSL5 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Reactivity human, mouse, rat
Cited Species mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACSL5 fusion protein Ag8258
Full Name acyl-CoA synthetase long-chain family member 5
Calculated molecular weight 76 kDa
Observed molecular weight 66-70 kDa
GenBank accession number BC007985
Gene symbol ACSL5
Gene ID (NCBI) 51703
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Acyl-CoA synthetase 5, a member of the ACSL gene family that catalyzes the activation of long-chain fatty acids for lipid biosynthesis, is the only ACSL isoform that is both, located on mitochondria and functionally involved in enterocyte apoptosis.ACSL5 could play a role in promoting fatty acid-induced lipoapoptosis in hepatocytes as important mechanism in fatty liver-related disorders(PMID:20470896). ACSL5 is most abundant in liver, brown adipose tissue, and intestine and is located on both the mitochondrial membrane and endoplasmic reticulum(PMID:20798351). It has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ACSL5 antibody 15708-1-APDownload protocol
IP protocol for ACSL5 antibody 15708-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Proteome Res

Integrated metabolomics and lipidomics analyses reveal metabolic reprogramming in human glioma with IDH1 mutation.

Authors - Lina Zhou
mouseWB

Sci Rep

Potent effects of dioscin against obesity in mice.

Authors - Min Liu
mouseWB

Food Chem

Effects of flavonoids from Rosa laevigata Michx fruit against high-fat diet-induced non-alcoholic fatty liver disease in rats.

Authors - Shuai Zhang
ratWB

J Biol Chem

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Authors - Lee G D Fryer
mouse, ratWB

Arch Toxicol

Protective effects of dioscin against alcohol-induced liver injury.

Authors - Xu Tingting T
mouseWB

Nat Commun

Tip60-mediated lipin 1 acetylation and ER translocation determine triacylglycerol synthesis rate.

Authors - Terytty Yang Li