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ACSS1 Polyclonal antibody
ACSS1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17138-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Caco-2 cells, Jurkat cells, mouse kidney tissue, RAW 264.7 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
17138-1-AP targets ACSS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ACSS1 fusion protein Ag10896 |
| Full Name | acyl-CoA synthetase short-chain family member 1 |
| Calculated Molecular Weight | 689 aa, 75 kDa |
| Observed Molecular Weight | 70-75 kDa |
| GenBank Accession Number | BC039261 |
| Gene Symbol | ACSS1 |
| Gene ID (NCBI) | 84532 |
| RRID | AB_2289182 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ACSS (acetyl-CoA synthetase) enzyme is the sole known mammalian enzyme that can catalyze the conversion of free acetate into acetyl coenzyme A (acetyl-CoA). The three known isoforms of human ACSS are termed ACSS1, ACSS2, and ACSS3. The main substrate of ACSS1 and ACSS2 is acetate, while the preferential substrate of ACSS3 is propionate. Two acetate related enzymes, ACSS1(GeneID: 84532) and ACSS2 (GeneID:55902) difer in their tissue distribution and subcellular localization. On the one hand, as a mitochondrial matrix enzyme, ACSS1 is expressed mainly in cardiac and skeletal muscle as well as brown adipose tissue. On the other hand, as a nuclear and cytoplasmic enzyme, ACSS2 is strongly expressed in the liver, kidney and heart and moderately expressed in the brain and testis.ACSS2 participates in lipid synthesis and facilitates protein acetylation by generating acetyl-CoA, while ACSS1 is involved in acetate oxidation. The functional diferences in these enzymes involve energy production through the tricarboxylic acid (TCA) cycle. Due to its more thorough utilization of intracellular acetate, ACSS2 is expressed in almost all cell types under diferent physiological conditions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| IHC protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| IP protocol for ACSS1 antibody 17138-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO J Sex-specific, reciprocal regulation of ERα and miR-22 controls muscle lipid metabolism in male mice. | ||
J Cell Physiol Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs.
| ||
PLoS Comput Biol Inferring latent temporal progression and regulatory networks from cross-sectional transcriptomic data of cancer samples. | ||
Front Pharmacol Integrated Metabolomics and Network Pharmacology Approach to Explain Possible Action Mechanisms of Xin-Sheng-Hua Granule for Treating Anemia. | ||
J Biol Chem Glucose-independent acetate metabolism promotes melanoma cell survival and tumor growth.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marina (Verified Customer) (12-18-2020) | I got different bands and none at 70kDa. I'm not sure if it is related to ACSS1 antibody quality, or if my cells don't express this protein...
 |
FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution
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