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ACTL7A Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 17355-1-AP

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Con: 34 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

ACTL7A Antibody WB mouse testis tissue 17355-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 17355-1-AP (ACTL7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

ACTL7A Antibody IF HepG2 cells 17355-1-AP

Immunofluorescent analysis of HepG2 cells, using ACTL7A antibody 17355-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

ACTL7A Antibody IHC mouse testis tissue 17355-1-AP

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ACTL7A Antibody IHC mouse testis tissue 17355-1-AP

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse testis tissue
Positive IHC detected in:
mouse testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:1000-1:5000
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ACTL7A fusion protein Ag10613
Full name:
actin-like 7A
Calculated molecular weight:
435aa,49 kDa
Observed molecular weight:
49 kDa
GenBank accession number:
BC014610
Gene ID (NCBI):
10881
Gene symbol
ACTL7A
Synonyms
Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

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