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ACTL7A Polyclonal antibody

ACTL7A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17355-1-AP

Synonyms

Actin like 7 alpha, actin like 7A, Actin like protein 7A, ACTL7A

Validation Data Gallery

  • WB analysis using 17355-1-AP

    WB analysis using 17355-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17355-1-AP (ACTL7A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 17355-1-AP

    IF Staining of HepG2 using 17355-1-AP

    Immunofluorescent analysis of HepG2 cells, using ACTL7A antibody 17355-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse testis using 17355-1-AP

    IHC staining of mouse testis using 17355-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17355-1-AP

    IHC staining of mouse testis using 17355-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17355-1-AP (ACTL7A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17355-1-AP targets ACTL7A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACTL7A fusion protein Ag10613
Full Name actin-like 7A
Calculated molecular weight 435 aa, 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC014610
Gene symbol ACTL7A
Gene ID (NCBI) 10881
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACTL7a is located in the acrosome and tail of mature spermatozoa and plays an important role in spermiogenesis. Recently mutation of ACTL7A has been linked to infertility.

Protocols

Product Specific Protocols
WB protocol for ACTL7A antibody 17355-1-APDownload protocol
IHC protocol for ACTL7A antibody 17355-1-APDownload protocol
IF protocol for ACTL7A antibody 17355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols