ACTL8 Polyclonal antibody

ACTL8 Polyclonal Antibody for WB, ELISA

Cat No. 17312-1-AP

Publications (1)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CT57, Cancer/testis antigen 57, Actin-like protein 8, Actin like protein 8, actin like 8

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWB

WB analysis using 17312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17312-1-AP (ACTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17312-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17312-1-AP (ACTL8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for ACTL8 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

A549 cells, Jurkat cells, HeLa cells, HepG2 cells, MOLT-4 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17312-1-AP targets ACTL8 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11281 Product name: Recombinant human ACTL8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC028909 Sequence: MASRTVIIDHGSGFLKAGTAGWNEPQMVFPNIVNYLPCKENPGPSYARRRVSLGIDICHPDTFSYPIERGRILNWEGVQYLWSFVLENHRREQEVPPVIITETPLREPADRKKMLEILFELLHVPSVLLADQLQMSLYASGLLTGVVVDSGYGLTRVQPFHQGRPLPASGKTLEFAGQDLSAYLLKSLFKEDCDRRCLFQLETVAVTQMNKCYVPQNLGEALDFRERQQSALDESNTYQLPDGSRVELTPMQRVAPEMFFSPQVFEQPGPSIPRAIVESVESCEISLRPLLVSHVMACGGNTLYPGFTKRLFRELMGDHVSSTKATVWEGSNRNFSVWLGASVVAHLSTYQSEWMSREEYGEHMRM Predict reactive species
Full Name	actin-like 8
Calculated Molecular Weight	366 aa, 41 kDa
Observed Molecular Weight	41 kDa
GenBank Accession Number	BC028909
Gene Symbol	ACTL8
Gene ID (NCBI)	81569
RRID	AB_2713917
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H568
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ACTL8 antibody 17312-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ACTL8 Polyclonal antibody (Cat# 17312-1-AP) has 1 total citation. It was cited in Molecular Cell (IF: 16, 2017) in a study titled "An Interaction Landscape of Ubiquitin Signaling," which falls within the research fields of ubiquitin signaling and protein–protein interaction mapping. The antibody was used for Western blot analysis in human samples.