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ADA Polyclonal antibody

ADA Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13328-1-AP

Synonyms

ADA, Adenosine aminohydrolase, adenosine deaminase

Validation Data Gallery

  • WB analysis of HeLa using 13328-1-AP

    WB analysis of HeLa using 13328-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13328-1-AP (ADA antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 13328-1-AP

    IHC staining of human stomach using 13328-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human stomach using 13328-1-AP

    IHC staining of human stomach using 13328-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 13328-1-AP (ADA Antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, Sp2/0 cells
Positive IHC detected inhuman stomach tissue, human cervical cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 13328-1-AP is ADA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADA fusion protein Ag4128
Full Name adenosine deaminase
Calculated molecular weight 363 aa, 41 kDa
Observed molecular weight 45-47 kDa
GenBank accession numberBC040226
Gene symbol ADA
Gene ID (NCBI) 100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ADA antibody 13328-1-APDownload protocol
IHC protocol for ADA antibody 13328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with NaCit antigen retrieval ph=6.

  • Applications: Immunohistochemistry,Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish brain (neurons) at 3dpf
ADA Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (neurons) at 3dpf (Cat no:13328-1-AP)