ADA Polyclonal antibody
ADA Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13328-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, Sp2/0 cells |
| Positive IHC detected in | human stomach tissue, human cervical cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Product Information
The immunogen of 13328-1-AP is ADA Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ADA fusion protein Ag4128 |
| Full Name | adenosine deaminase |
| Calculated molecular weight | 363 aa, 41 kDa |
| Observed molecular weight | 45-47 kDa |
| GenBank accession number | BC040226 |
| Gene symbol | ADA |
| Gene ID (NCBI) | 100 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ADA antibody 13328-1-AP | Download protocol |
| IHC protocol for ADA antibody 13328-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryan (Verified Customer) (02-28-2019) | Tissue was fixed in PFA with NaCit antigen retrieval ph=6.
 |
