ADAM12 Antibody

ADAM12 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat brain

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26928-1-AP

Synonyms

ADAM 12, ADAM12, MCMP, MCMPMltna, Meltrin alpha, MLTN, MLTNA



Tested Applications

Positive WB detected inmouse brain tissue, A431 cells, A549 cells, rat brain tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26928-1-AP immunogen is Fusion Protein ADAM12 protein expressed in E. coli.

Reactivity human, mouse, rat brain
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM12 fusion protein Ag17474
Full Name ADAM metallopeptidase domain 12
Calculated molecular weight 909aa,100 kDa
Observed molecular weight 68 kDa
GenBank accession number BC060804
Gene symbol ADAM12
Gene ID (NCBI) 8038
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAM12, also named as MLTN and Meltrin-alpha, is involved in skeletal muscle regeneration, specifically at the onset of cell fusion. It is also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors. ADAM12 is expressed in human malignant tumors. It is involved in the regulation of growth factor activities and integrin functions, leading to promotion of cell growth and invasion, although the precise mechanisms of these are not clear at the present time.(PMID:17355265) ADAM12’s ability to degrade extracellular matrix components likely allows it to detach cancer cells from the basement membrane and assist them on their route to metastasis. But the protein’s role not just as a biomarker of breast cancer but as a gateway to cancer cell migration is only now being understood. ADAM12 has 2 isoforms with the calculated molecular mass of 100 and 80 kDa, and this antibody can recognize all isoforms of ADAM12. ADAM12 has 4 forms: 120kd full-length form, 90kd mature (processed form that lack the prodomain); 50-68kd degradation product and 27kd prodomain.

Protocols

Product Specific Protocols
WB protocol for ADAM12 antibody 26928-1-APDownload protocol
IHC protocol for ADAM12 antibody 26928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols