ADAM19 Polyclonal antibody

ADAM19 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22216-1-AP

Synonyms

ADAM 19, ADAM19, FKSG34, MADDAM, Meltrin beta, MLTNB



Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman placenta tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22216-1-AP is ADAM19 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAM19 fusion protein Ag17544
Full Name ADAM metallopeptidase domain 19 (meltrin beta)
Calculated molecular weight 955 aa, 105 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC024214
Gene symbol ADAM19
Gene ID (NCBI) 8728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Meltrinβ(ADAM19) is a metalloprotease-disintegrin expressed in the peripheral nervous system and other organs during embryogenesis. Its specific patterns of expression during embryogenesis suggest that it is involved in the development of the peripheral nervous system, skeletal muscle, bone and heart tissue(PMID:12482604). It participates in the proteolytic processing of beta-type neuregulin isoforms which are involved in neurogenesis and synaptogenesis, suggesting a regulatory role in glial cell.

Protocols

Product Specific Protocols
WB protocol for ADAM19 antibody 22216-1-APDownload protocol
IHC protocol for ADAM19 antibody 22216-1-APDownload protocol
IP protocol for ADAM19 antibody 22216-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols