ADAT2 Polyclonal antibody

ADAT2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13621-1-AP

Synonyms

ADAT2, DEADC1, dJ20N2, TAD2



Tested Applications

Positive WB detected inA431 cells, mouse brain tissue, mouse pancreas tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13621-1-AP targets ADAT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADAT2 fusion protein Ag4529
Full Name adenosine deaminase, tRNA-specific 2, TAD2 homolog (S. cerevisiae)
Calculated molecular weight 191 aa, 21 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC037955
Gene symbol ADAT2
Gene ID (NCBI) 134637
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ADAT2(tRNA-specific adenosine deaminase 2) is also named as DEADC1 and Belongs to the cytidine and deoxycytidylate deaminase family. It is a 24-kDa molecular mass protein that harbors all the conserved motifs required for deamination. ADAT2 and ADAT3 can function as a heterodimer which catalyses inosine formation at the wobble position (position 34) in eukaryotic tRNAs(PMID:12457566). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ADAT2 antibody 13621-1-APDownload protocol
IHC protocol for ADAT2 antibody 13621-1-APDownload protocol
IF protocol for ADAT2 antibody 13621-1-APDownload protocol
IP protocol for ADAT2 antibody 13621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols